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Analysis of intermediate structure in proton pump mechanism of proteorhodopsin

Research Project

Project/Area Number 15K18523
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Hosaka Toshiaki  国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 研究員 (40462725)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsロドプシン / X線結晶構造解析
Outline of Final Research Achievements

Proteorhodopsin (PR) is one of microbial-type rhodopsins such as bacteriorhodopsin (BR) and functions as a light-driven proton pump. PR-bearing bacteria are widely found among microorganisms in the sea and fresh water. In this study, we aimed for structural analysis of these PRs. PRs were prepared and purified from E. coli cell-free synthesis system and crystallized by LCP method. I presented here (1) two types of PR crystal structure that absorb light at 490 nm and 530 nm, (2) radiation damage-free PR structure, and (3) light absorption-mutant structure of PR. In particular, the structure revealed that the interaction of the amino acid and a water molecule which related to light absorption causes structural changes of retinal.
I participated in the structural analysis of BR intermediate at SACLA and also reported structural analysis of light-driven Cl--pumping rhodopsin that is a homolog of PRs.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2016

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Book (1 results)

  • [Journal Article] Structural mechanism for light-driven transport by a new type of chloride ion pump, Nonlabens marinus rhodopsin-32016

    • Author(s)
      Toshiaki Hosaka, Susumu Yoshizawa, Yu Nakajima, Noboru Ohsawa, Masakatsu Hato, Edward F. DeLong, Kazuhiro Kogure, Shigeyuki Yokoyama, Tomomi Kimura-Someya, Wataru Iwasaki and Mikako Shirouzu
    • Journal Title

      Journal of Biological Chemistry

      Volume: 291 Issue: 34 Pages: 17488-17495

    • DOI

      10.1074/jbc.m116.728220

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A three dimensional movie of structural changes in bacteriorhodopsin.2016

    • Author(s)
      Nango, E., Royant, A.†, Kubo, M.† et al. (†equal contribution)(45名中2番目)
    • Journal Title

      Science

      Volume: 354 Issue: 6319 Pages: 1552-1557

    • DOI

      10.1126/science.aah3497

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Book] Advanced Methods in Structural Biology2016

    • Author(s)
      T. Kimura-Someya, T. Hosaka, T. Shinoda, K. Shimono, M. Shirouzu, S. Yokoyama
    • Total Pages
      340
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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