| Project/Area Number |
15K18527
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | International Christian University |
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Principal Investigator |
Ito Emi 国際基督教大学, 教養学部, 特任助教 (80726422)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | RAB GTPases / Rho GTPases / Rho of Plant (Rop) / RAB5 / エフェクター / AtSWAP70 / 細胞生物学 / 植物細胞 / RAB GTPase / Effector / 細胞内シグナリング / Rac/Rop |
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| Outline of Final Research Achievements |
I have identified SWAP70 as a novel effector protein of plant RAB5 GTPases. Since SWAP70 is known as a Rac activation factor in other eukaryotic systems, I hypothesized that RAB5 and Rho of Plant GTPases (ROP; counterpart of Rho in plants) constitute a signaling cascade via SWAP70. To test this hypothesis, I cloned Rop members, and tested their interaction with SWAP70. As a result, ROP7 was the only member capable for binding to SWAP70. Next, I examined the effect of SWAP70 and RAB5 members on the subcellular localization of ROP7. ROP7 mainly localized to the plasma membrane, nucleus and nucleolus-like structures in tobacco leaf epidermal cells. When SWAP70 or RAB5s were co-expressed with ROP7, subpopulation of ROP7 shifted to localize to the endosomes. ROP7 is known to show tissue-specific expression patterns. Thus, my results suggest that plant RAB5s trigger tissue-specific phenomena by regulating the subcellular functions of ROP7 through SWAP70.
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