Screening of transport component of ATF6 by haploid medaka system

• Project/Area Number: 15K18529
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Kyoto University
• Principal Investigator: Tokiro Ishikawa 京都大学, 理学研究科, 助教 (70632545)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 小胞体ストレス応答 / 小胞体ストレス / UPR / メダカ / ATF6

Outline of Final Research Achievements

ATF6 is a transcription factor that regulates the unfolded protein response. In response to ER stress, ATF6 is transported from the ER to the Golgi apparatus and activated. The transport factor involved in the precess has not been identified. To understand activation mechanisms of ATF6, I was trying to identify the transport factor by the new forward genetic screening system using the hybrid medaka embryo.
I found that the rate of generation of hybrid embryo is lower than expected. I continued to improve this systems, and started establishing alternative screening system using mutant fish in which hyper recombination occurs.

Research Products

• [Journal Article] UPR Transducer BBF2H7 Allows Export of Type II Collagen in a Cargo- and Developmental Stage-Specific Manner (2017)
  • Author(s): Tokiro Ishikawa, Takuya Toyama, Yuki Nakamura, Kentaro Tamada, Hitomi Shimizu, Satoshi Ninagawa, Tetsuya Okada, Yasuhiro Kamei, Tomoko Ishikawa-Fujiwara, Takeshi Todo, Eriko Aoyama, Masaharu Takigawa, Akihiro Harada and Kazutoshi Mori
  • Journal Title: Journal of cell biology Volume: 印刷中 Issue: 6 Pages: 1761-1774
  • DOI: 10.1083/jcb.201609100
  • NAID: 120006343784
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway. (2015)
  • Author(s): Ninagawa S, Okada T, Sumitomo Y, Horimoto S, Sugimoto T, Ishikawa T, Takeda S, Yamamoto T, Suzuki T, Kamiya Y, Kato K, Mori K.
  • Journal Title: The Journal of Cellular Biology Volume: 211 Issue: 4 Pages: 775-784
  • DOI: 10.1083/jcb.201504109
  • NAID: 120005672576
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Comprehensive Analysis of IRE1 Mediated UPR Pathway in Medaka Fish (2016)
  • Author(s): Tokiro Ishikawa, Tetsuya Okada, Tomoko Ishikawa-Fujiwara, Satoshi Ansai, Yasuhiro Kamei, Masato Kinoshita, Takeshi Todo, Kazutoshi Mori
  • Organizer: International Meeting on Aquatic Model Organisms for Human Disease and Toxicology Research
  • Place of Presentation: 岡崎
  • Year and Date: 2016-03-18
  • Int'l Joint Research
• [Presentation] Comprehensive analysis of the IRE1 pathway during development of medaka fish (2015)
  • Author(s): Tokiro Ishikawa, Tetsuya Okada, Tomoko Ishikawa-Fujiwara, Satoshi Ansai, Yasuhiro Kamei, Masato Kinoshita, Takeshi Todo, Kazutoshi Mori
  • Organizer: BMB2015
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] phiC31インテグレースによる高効率遺伝子導入に用いる Landing site(attP) 導入メダカ系統の樹立 (2015)
  • Author(s): 石川 時郎, 安齋 賢, 岡田 徹也, 木下 政人, 森 和俊
  • Organizer: the 21st Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 岡崎
  • Year and Date: 2015-09-19
• [Presentation] メダカ発生過程におけるIRE1経路の生理的機能の包括的解析 (2015)
  • Author(s): 石川 時郎, 岡田 徹也, 藤原-石川 智子, 安齋 賢, 亀井 保博, 木下 政人, 藤堂 剛, 森 和俊
  • Organizer: the 21st Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 岡崎
  • Year and Date: 2015-09-19