Ragulatory mechanism of selective autophagy

• Project/Area Number: 15K18530
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Yamaguchi University
• Principal Investigator: Shibutani Shusaku 山口大学, 共同獣医学部, 助教(テニュアトラック) (20534473)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Atg / オートファジー / 選択的オートファジー / ミトコンドリア / マイトファジー

Outline of Final Research Achievements

Selective autophagy is thought to maintain intracellular homeostasis by eliminating unwanted/dangerous materials. Although it has been shown that Atg proteins accumulate on the substrate in selective autophagy, events that happen afterwards are not clear. In this study, we discovered that the forced recruitment of LC3 to mitochondria is sufficient to induce mitochondrial degradation. We hypothesize that mitochondrial fission is induced at the contact site with the isolation membrane, which later engulfs the mitochondrial fragment generated by the fission. Moreover, this study suggests the existence of unidentified mitochondrial fission factors responsible for the mitochondrial fission during selective autophagy of mitochondria.

Research Products

• [Journal Article] Bosutinib, an SRC inhibitor, induces caspase-independent cell death associated with permeabilization of lysosomal membranes in melanoma cells. (2017)
  • Author(s): Noguchi S, Shibutani S, Fukushima K, Mori T, Igase M, Mizuno T.
  • Journal Title: Vet Comp Oncol Volume: 印刷中 Issue: 1 Pages: 69-76
  • DOI: 10.1111/vco.12312
  • Peer Reviewed
• [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (2016)
  • Author(s): Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Acevedo Arozena A, Adachi H, Adams CM, Adams PD, Adeli K, Adhihetty PJ, Adler SG, Agam G, Agarwal R, Aghi MK, Agnello M, Agostinis P, Aguilar PV, Aguirre-Ghiso J, Shibutani S. et al.
  • Journal Title: Autophagy Volume: 12 Pages: 1-222
  • Int'l Joint Research
• [Journal Article] The requirement of environmental acidification for Ibaraki virus infection to host cells (2016)
  • Author(s): Tsuruta Y, Shibutani ST, Watanabe R, Iwata H
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 1 Pages: 153-156
  • DOI: 10.1292/jvms.15-0222
  • NAID: 130005121588
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Autophagy and autophagy-related proteins in the immune system. (2015)
  • Author(s): Shibutani ST, Saitoh T, Nowag H, Munz C, Yoshimori T
  • Journal Title: Nat Immunoｌ Volume: 16 Issue: 10 Pages: 1014-1024
  • DOI: 10.1038/ni.3273
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 栄養状態をモニターし細胞増殖を制御するmTORC1の活性化機構 (2017)
  • Author(s): Shibutani S
  • Organizer: 山口大学研究推進体「小動物のガンに対するトランスレーショナル研究ユニット」キックオフシンポジウム2017
  • Place of Presentation: 山口大学（山口県山口市）
• [Presentation] イバラキウイルスの哺乳動物細胞侵入経路の解析 (2016)
  • Author(s): Tsuruta Y, Shibutani S, Watanabe R, Iwata H
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
  • Year and Date: 2016-09-05
• [Presentation] 胃癌におけるProtein Phosphatase 6の役割の解明 (2016)
  • Author(s): Fujiwara N, Shibutani S, Saitoh T, Akira S, Usui T, Ohama T, Sato K
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
  • Year and Date: 2016-09-05
• [Presentation] Membrane traffic as defense against intracellular dangers (2016)
  • Author(s): Shibutani S
  • Organizer: International Symposium on Intracellular Pathogens, Yamaguchi 2016
  • Place of Presentation: 山口大学（山口県、山口市）
  • Year and Date: 2016-02-05
  • Int'l Joint Research / Invited
• [Patent(Industrial Property Rights)] ｍTORC1活性化抑制剤 (2016)
  • Inventor(s): 渋谷周作、岩田祐之
  • Industrial Property Rights Holder: 国立大学法人山口大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-206578
  • Filing Date: 2016-10-21