| Project/Area Number |
15K18531
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Kyushu University |
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Principal Investigator |
Okada Satoshi 九州大学, 医学研究院, 助教 (30734488)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 出芽酵母 / 細胞老化 / オルガネラ / イメージング / 胞子形成 / 細胞分裂寿命 |
|---|
| Outline of Final Research Achievements |
Budding yeast strains in which ageing-related organelles are visualized by C-terminal tagging with green or red fluorescent protein were constructed. Live cell imaging conditions for these strains were optimized. To achieve long-term time-lapse live cell imaging, a microfluidics-based stage top cell culture device was introduced on a microscope. On the device, cell culture conditions for high efficiency sporulation induction were optimized. By development of BiFC system of novel bright fluorescent protein, RENT complexes containing an ageing-related factor, Sir2, were successfully visualized in living yeast cells.
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