| Project/Area Number |
15K18533
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Kurume University |
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Principal Investigator |
Toyoda Yusuke 久留米大学, 分子生命科学研究所, 助教 (10587653)
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| Research Collaborator |
SAITOH Shigeaki 久留米大学, 分子生命科学研究所, 教授
YANAGIDA Mitsuhiro 沖縄科学技術大学院大学, GOユニット, 教授
SAJIKI Kenichi 沖縄科学技術大学院大学, GOユニット, 研究員
Ozlu Nurhan Koc University, Assistant Professor
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 低グルコース環境 / Ght5 / LGS変異体 / グルコース輸送体 / GLUT1 / GRP78 / シグナル伝達 / 細胞増殖 / グルコース / グルコース枯渇 / 低グルコース / 糖輸送体 / リン酸化 / TORC2 / Ksg1 / lgs / 分裂酵母 / 細胞内局在 |
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| Outline of Final Research Achievements |
Proper regulations of glucose uptake via glucose transporters are not only vital for cellular proliferation but also related to human diseases including type II diabetes and cancer. This study was focused on the molecular mechanism that ensures cell-surface targeting of the fission yeast glucose transporter Ght5, which is essential for vigorous cell proliferation under glucose-limited conditions. Out of the 51 genes tested, 25 genes involved in vesicle transport or signal transduction functioned in proliferation under glucose-limited conditions by ensuring cell-surface localization of Ght5-GFP. In human cultured cells, endoplasmic reticulum chaperones such as GRP78, which were required for cell-surface localization of GLUT1 under glucose-rich conditions, became dispensable upon glucose starvation. Taken together, this study uncovered that the transport mechanism for cell-surface multi-pass transmembrane proteins is modulated by the extracellular glucose level.
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