| Project/Area Number |
15K18539
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kanazawa University (2016)
Hokkaido University (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 始原生殖細胞 / 胚性幹細胞 / 転写因子 / エピジェネティクス / Nanog / マウス / 幹細胞 |
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| Outline of Final Research Achievements |
The decline of birth rate in developed countries including Japan is one of urgent issues. But the research on In Vitro germ cell induction which could be a fundamental solution to the problem has been just started.
In this study, I combined the Primordial Germ Cell-Like Cell (PGCLC) induction technique and the immunoprecipitation - mass spectrometry analysis to perform extensive analyses and tried to understand the mechanism which regulate germ cell development in cooperation with transcription factors. Furthermore, by using the CRISPR/Cas9 technique, I disrupted candidate genes and genomic regions which were bound by transcription factors and performed verification experiments in the context of PGCLC.
As a result, the details of the interaction among transcription factors and epigenome which are essential for the germ cell development has been clarified.
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