Elucidation of a force-dependent mechanism for maintaining radial size of epithelial tubes

• Project/Area Number: 15K18541
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: Hirashima Tsuyoshi 京都大学, 医学研究科, 講師 (10620198)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: サイズ維持 / 上皮管 / 形態形成 / 数理モデル / イメージング / 器官形成 / 精巣上体 / メカノバイオロジー / バーテクスモデル / 蛍光ライブイメージング / バーテクスダイナミクスモデル / ライブイメージング / 多細胞力学応答 / 多細胞ライブイメージング / 多細胞動態の定量 / 多細胞動態の数理モデル

Outline of Final Research Achievements

For the understanding of mechanisms for size maintenance of biological tissues, I focused on an aspect of mechanics in mouse epididymal tubules during developmental process. I aimed at clarifying collective multicellular behaviors for maintaining diameter of epididymal tubules by using quantitative fluorescence imaging and mathematical modeling. The integrated approach revealed a mechanism of the size maintenance that constitutive cells within the tubules receive mechanical forces and respond by active cellular movement.

Research Products

• [Int'l Joint Research] Leiden University(オランダ)
• [Journal Article] Cellular Potts Modeling of Complex Multicellular Behaviors in Tissue Morphogenesis (2017)
  • Author(s): Tsuyoshi Hirashima, Elisabeth G. Rens, and Roeland M.H. Merks
  • Journal Title: Development, Growth & Differentiation Volume: 59 Issue: 5 Pages: 329-339
  • DOI: 10.1111/dgd.12358
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] In vitro tubulogenesis of Madin-Darby canine kidney (MDCK) spheroids occurs depending on constituent cell number and scaffold gel concentration (2017)
  • Author(s): Tsuyoshi Hirashima, Miho Hoshuyama, and Taiji Adachi
  • Journal Title: Journal of Theoretical Biology Volume: 435 Pages: 110-115
  • DOI: 10.1016/j.jtbi.2017.09.009
  • Peer Reviewed
• [Journal Article] Mathematical Study on Robust Tissue Pattern Formation in Growing Epididymal Tubule (2016)
  • Author(s): Hirashima Tsuyoshi
  • Journal Title: Journal of Theoretical Biology Volume: 407 Pages: 71-80
  • DOI: 10.1016/j.jtbi.2016.07.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 上皮管組織の異方的な成長を支える協調的な多細胞動態と力学 (2016)
  • Author(s): 平島剛志
  • Journal Title: 生体の科学 Volume: 67
• [Journal Article] Procedures for the Quantification of Whole-Tissue Immunofluorescence Images Obtained at Single-Cell Resolution during Murine Tubular Organ Development (2015)
  • Author(s): Tsuyoshi Hirashima and Taiji Adachi
  • Journal Title: PLOS ONE Volume: 10 Issue: 8 Pages: e0135343-e0135343
  • DOI: 10.1371/journal.pone.0135343
  • NAID: 120005678155
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 多細胞動態・形態の定量による恒常的な管径維持システムの解明 (2017)
  • Author(s): 平島剛志
  • Organizer: 定量生物の会第８回年会
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2017-01-08
  • Invited
• [Presentation] Adaptive Multicellular Behaviors as Mechanoresponse for Radial Size Maintenance of Epithelial Tube (2017)
  • Author(s): Tsuyoshi Hirashima and Taiji Adachi
  • Organizer: 18th International Congress of Developmental Biology
  • Int'l Joint Research
• [Presentation] Anisotropic Cellular Mechanoresponse Maintains the Radial Size of Developing Epididymal Tubules (2017)
  • Author(s): 平島剛志、安達泰治
  • Organizer: 第55回日本生物物理学会
• [Presentation] 上皮管の形態形成に働く細胞の力学応答システム (2017)
  • Author(s): 平島剛志、安達泰治
  • Organizer: 第69回日本細胞生物学会大会
  • Invited
• [Presentation] How to encounter endothelial cells in deep tissues - mechanical interaction between endothelial cells and epithelial tube. (2017)
  • Author(s): Tsuyoshi Hirashima amd Taiji Adachi
  • Organizer: 第50回日本発生生物学会年
• [Presentation] 生体外培養下における管形成の誘導について (2017)
  • Author(s): 平島剛志、寳珠山美歩、安達泰治
  • Organizer: 日本メカノバイオロジー学会第２回
• [Presentation] 増殖する上皮管の径サイズ恒常維持に働く適応的な多細胞動態システム (2016)
  • Author(s): 平島剛志、安達泰治
  • Organizer: 日本発生生物学会秋季シンポジウム2016
  • Place of Presentation: 三島市民文化会館
  • Year and Date: 2016-10-19
• [Presentation] Adaptive Multicellular Dynamics via Directional Mechanosensing for Homeostatic Radial Size of Proliferating Tube (2016)
  • Author(s): Tsuyoshi Hirashima and Taiji Adachi
  • Organizer: Mechanobiology from molecules to tissues, theory and applications
  • Place of Presentation: Quy Nhon, Vietnam
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] 増殖する上皮管の径サイズ恒常維持に働く適応的な多細胞動態システムについて (2016)
  • Author(s): 平島剛志、安達泰治
  • Organizer: 第68回日本細胞生物学会・日本ケミカルバイオロジー学会第11回年回 合同大会
  • Place of Presentation: 京都テルサ
  • Year and Date: 2016-06-14
  • Invited
• [Presentation] Adaptive Cellular Behavior for Homeostatic Radial Size in Proliferating Tube (2016)
  • Author(s): Tsuyoshi Hirashima and Taiji Adachi
  • Organizer: RIKEN CDB Symposium 2016- Size in Development
  • Place of Presentation: 理研CDB、神戸
  • Year and Date: 2016-03-28
  • Int'l Joint Research
• [Presentation] 臓器の形態形成を支える細胞動態機構の解明に向けて (2016)
  • Author(s): 平島剛志、安達泰治
  • Organizer: 生命動態システム科学四拠点・CREST・PREST合同シンポジウム
  • Place of Presentation: シャラトンホテル広島
  • Year and Date: 2016-03-25
• [Presentation] 組織の異方成長に関与する新たな細胞力覚システム (2016)
  • Author(s): 平島剛志、安達泰治
  • Organizer: 第１回日本メカノバイオロジー学会学術大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2016-03-01
• [Presentation] Adaptive Cellular Behaviors Realizing Homeostatic Radial Size in Developing Epithelial Tubule (2015)
  • Author(s): Tsuyoshi Hirashima and Taiji Adachi
  • Organizer: Commemorative Symposium for the 31st International Prize for Biology
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] On Dynamic Cellular Behaviors Realizing Homeostatic Radial Size in Developing Epithelial Tubule (2015)
  • Author(s): Tsuyoshi Hirashima and Taiji Adachi
  • Organizer: EMBL symposium - Physics of Cells and Tissues
  • Place of Presentation: EMBL, Heidelberg
  • Year and Date: 2015-09-30
  • Int'l Joint Research
• [Presentation] Cellular Surface Dynamics of Biological Tube for Homeostatic Radial Size (2015)
  • Author(s): Tsuyoshi Hirashima and Taiji Adachi
  • Organizer: iCeMS International Symposium - Hierarchical Dynamics in Soft Materials and Biological Matter
  • Place of Presentation: 京都大学
  • Year and Date: 2015-09-24
  • Int'l Joint Research
• [Presentation] On Dynamic Cellular Behaviors Realizing Stable Radial Size in Developing Epithelial Tubule (2015)
  • Author(s): Tsuyoshi Hirashima and Taiji Adachi
  • Organizer: 第53回日本生物物理学会
  • Place of Presentation: 金沢大学
  • Year and Date: 2015-09-14
• [Presentation] Modeling Apical Surface Dynamics in Elongating Tubule Morphogenesis (2015)
  • Author(s): Tsuyoshi Hirashima and Taiji Adachi
  • Organizer: 2015年日本数理生物学会/日中韓数理生物学コロキウム合同大会
  • Place of Presentation: 同志社大学
  • Year and Date: 2015-08-26
• [Presentation] 伸びてねじれる精巣上体細管のかたち作り (2015)
  • Author(s): 平島剛志
  • Organizer: 第79回形の科学会シンポジウム「生物にみられるねじれ構造」
  • Place of Presentation: 千葉工業大学
  • Year and Date: 2015-06-13
  • Invited
• [Presentation] On a Mechanism of Adaptive Cellular Behavior Achieving Stable Radial Size of a Developing Epithelial Tubule (2015)
  • Author(s): Tsuyoshi Hirashima and Taiji Adachi
  • Organizer: 第48回日本発生生物学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2015-06-03
• [Remarks] 研究者のホームページ
  • URL: https://sites.google.com/site/tsuyoshihirashima/home