| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Fertilization calcium waves are a conserved signal that triggers both animal and plant embryonic development. The calcium waves are thought to be propagated by the action of the IP3 receptor calcium channels. However, in the nematode C. elegans, involvement of a novel channel is suggested. In this study, we aimed to identify calcium channels that are responsible for the propagation of the calcium waves. We generated a transgenic C. elegans strain that expresses the genetically-encoded calcium indicator GCaMP6s in the germline. By using this transgenic strain, we conducted a feeding RNA interference screen. We identified several candidate genes that might be involved in the calcium wave propagation.
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