Functional analysis of GLES1 essential for chloroplast development in guard cells

• Project/Area Number: 15K18556
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyushu University
• Principal Investigator: NEGI JUNTARO 九州大学, 理学研究院, 准教授 (70529099)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 気孔 / 葉緑体 / 脂質 / 脂質代謝 / CO2

Outline of Final Research Achievements

Stomatal guard cells develop unique chloroplasts in land plant species. However, the developmental mechanisms and function of chloroplasts in guard cells remain unclear. In seed plants, chloroplast membrane lipids are synthesized via two pathways: the prokaryotic and the eukaryotic pathways. We gained insight into these pathways by isolating and examining an Arabidopsis mutant, gles1, which has achlorophyllous stomata and impaired stomatal responses to CO2 and light. The GLES1 gene encodes a putative regulatory component of the trigalactosyldiacylglycerol protein complex that mediates the ER-to-chloroplast lipid transport via the eukaryotic pathway. Lipidomic analysis reveals that in wild type, the prokaryotic pathway is dysfunctional specifically in guard cells, whereas in gles1 guard cells, the eukaryotic pathway is also abrogated. In conclusion, the eukaryotic lipid pathway is essential for guard-cell chloroplasts to develop CO2 and light signal sensing machinery.

Research Products

• [Int'l Joint Research] カリフォルニア大学サンディエゴ校(U.S.A.)
• [Int'l Joint Research] カリフォルニア大学サンディエゴ校(U.S.A.)
• [Int'l Joint Research] University of California at San Diego(U.S.A.)
• [Journal Article] Differential Effects of Phosphatidylinositol 4-Kinase (PI4K) and 3-Kinase (PI3K) Inhibitors on Stomatal Responses to Environmental Signals. (2017)
  • Author(s): Takahashi, S., Monda, K., Higaki, T., Negi, J., Hashimoto-Sugimoto, M., Hasezawa, S. and Iba, K.
  • Journal Title: Front. Plant Sci. Volume: 8 Pages: 677-677
  • DOI: 10.3389/fpls.2017.00677
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Contribution of the S-type anion channel SLAC1 to stomatal control and its dependence on developmental stage in rice. (2017)
  • Author(s): Kusumi, K., Hashimura, A., Yamamoto, Y., Negi, J. and Iba, K.
  • Journal Title: Plant Cell Physiol. Volume: 58 Issue: 12 Pages: 2085-2094
  • DOI: 10.1093/pcp/pcx142
  • Peer Reviewed
• [Journal Article] サーマルイメージング法による気孔CO2応答解析から見出された新たな植物環境応答機構 (2017)
  • Author(s): 高橋 將、門田 慧奈、祢冝 淳太郎、射場 厚
  • Journal Title: 光合成研究 Volume: 27 Pages: 84-93
  • Peer Reviewed
• [Journal Article] Differential effects of phosphatidylinositol 4-Kinase (PI4K) and 3-Kinase (PI3K) Inhibitors on stomatal responses to environmental signals (2017)
  • Author(s): Takahashi, S., Monda, K., Higaki, T., Negi, J., Hashimoto-Sugimoto, M., Hasezawa, S., Iba, K.
  • Journal Title: Frontiers in Plant Science Volume: 未定
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Dominant and recessive mutations in the Raf-like kinase HT1 gene completely disrupt stomatal responses to CO2. (2016)
  • Author(s): Hashimoto-Sugimoto, M., Negi, J., Monda, K., Higaki, T., Isogai, Y., Nakano, T., Hasezawa, S. and Iba, K.
  • Journal Title: J.Exp. Bot. Volume: 未定 Issue: 11 Pages: 3251-3261
  • DOI: 10.1093/jxb/erw134
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CO2 sensing and stomatal conductance regulation: advances and open questions. (2016)
  • Author(s): Engineer, C., Hashimoto-Sugimoto, M., Negi, J., Rappel, W-J., Iba, K. and Schroeder, J.
  • Journal Title: Trends Plant Sci. Volume: 21 Issue: 1 Pages: 16-30
  • DOI: 10.1016/j.tplants.2015.08.014
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The transmembrane region of guard cell SLAC1 channels perceives CO2 signals via an ABA-independent pathway in Arabidopsis. (2016)
  • Author(s): Yamamoto, Y., Negi, J., Wang, C., Isogai, Y., Schroeder, J.I. and Iba, K.
  • Journal Title: Plant Cell Volume: 28 Pages: 557-567
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Enhanced stomatal conductance by a spontaneous Arabidopsis tetraploid, Me-0, results from increased stomatal size and greater stomatal aperture. (2016)
  • Author(s): Monda, K., Araki, H., Kuhara, S., Ishigaki, G., Akashi, R., Negi, J., Kojima M., Sakakibara, H., Takahashi, S., Hashimoto-Sugimoto, M., Goto, N. and Iba, K.
  • Journal Title: Plant Physiol. Volume: 170 Pages: 1435-1444
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 炭素栄養シグナルとしてのCO2による気孔制御 (2017)
  • Author(s): 祢冝 淳太郎
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
  • Invited
• [Presentation] 孔辺細胞と葉肉細胞の脂質組成の違い (2017)
  • Author(s): 多田隈 遼亮、宋 普錫、楠見 健介、西田 生郎、射場 厚、祢冝 淳太郎
  • Organizer: 第35回 日本植物細胞分子生物学会 大会
• [Presentation] 気孔の葉緑体形成には真核型経路による脂質合成が必須である (2017)
  • Author(s): 祢冝 淳太郎、宋 普錫、多田隈 遼亮、宗正 晋太郎、藤田 麻友美、楠見 健介、Julian Schroeder、射場 厚
  • Organizer: 日本植物学会 第81回大会
• [Presentation] 真核型の脂質代謝経路は気孔の葉緑体形成およびCO2応答に必須である (2017)
  • Author(s): 祢冝 淳太郎、宋 普錫、多田隈 遼亮、宗正 晋太郎、藤田 麻友美、楠見 健介、Julian Schroeder、 西田 生郎、射場 厚
  • Organizer: 第59回 日本植物生理学会年会
• [Presentation] 孔辺細胞におけるフォスファチジルエタノールアミン合成酵素PECT1の役割 (2016)
  • Author(s): 袮冝 淳太郎、小野 勇兵、岡部 誠、星野 奈摘、西田 生郎、射場 厚
  • Organizer: 第34回日本植物細胞分子生物学会
  • Place of Presentation: 信州大学繊維学部
  • Year and Date: 2016-09-01
• [Presentation] A rate-limiting enzyme in phosphatidylethanolamine biosynthesis, PECT1, is involved in the stomatal regulation in Arabidopsis (2016)
  • Author(s): Negi, J., Okabe, M., Ono, Y., Hoshino, N., Nishida, I., Iba, K.
  • Organizer: International Conference on Arabidopsis Research 2016
  • Place of Presentation: GyeongJu (KOREA)
  • Year and Date: 2016-06-29
  • Int'l Joint Research
• [Presentation] A rate-limiting enzyme in phosphatidylethanolamine biosynthesis, PECT1, is involved in the stomatal movement in Arabidopsis (2016)
  • Author(s): Juntaro Negi, Makoto Okabe, Yuhei Ono, Natsumi Hoshino, Ikuo Nishida, Koh Iba
  • Organizer: 第57回日本植物生理学会
  • Place of Presentation: 盛岡（岩手大学）
  • Year and Date: 2016-03-18
• [Presentation] 気孔の環境応答及び形成の分子機構の解明 (2015)
  • Author(s): 祢冝淳太郎
  • Organizer: 第33回日本植物細胞分子生物学会
  • Place of Presentation: 東京（東京大学）
  • Year and Date: 2015-08-10
  • Invited
• [Presentation] 気孔葉緑体変異体gles1はCO2によるS型陰イオンチャネル活性制御に異常を持つ (2015)
  • Author(s): 祢冝淳太郎, 楠見健介, 宗正晋太郎, 藤田麻友美, Julian Schroeder, 射場 厚
  • Organizer: 第33回日本植物細胞分子生物学会
  • Place of Presentation: 東京（東京大学）
  • Year and Date: 2015-08-10
• [Presentation] CO2 activation of the guard cell S-type anion channel is impaired in an Arabidopsis mutant with non-chlorophyllous stomata (2015)
  • Author(s): Juntaro Negi, Kensuke Kusumi, Shintaro Munemasa, Mayumi Fujita, Julian Schroeder, Koh Iba
  • Organizer: 26th International Conference on Arabidopsis Research
  • Place of Presentation: Paris, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Remarks] HP
  • URL: http://www.biology.kyushu-u.ac.jp/~plant/index.html
• [Remarks]
  • URL: http://www.biology.kyushu-u.ac.jp/~plant/
• [Remarks] 九州大学大学院・理学研究院・植物生理学講座
  • URL: http://www.biology.kyushu-u.ac.jp/~plant/