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Role of a novel DNA demethylation enzyme in cellular memory

Research Project

Project/Area Number 15K18558
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionUniversity of Tsukuba

Principal Investigator

Buzas Diana Mihaela  筑波大学, 生命環境系, 准教授 (00616229)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsDNA demethylation / DNA methylation / methylation / Epigenetics
Outline of Final Research Achievements

Some gene expression programs induced by transient signals remain stable across cell divisions. Such cellular memory may replay on the stable yet reversible DNA modification 5 cytosine methylation (5mC). We identified DRE2 as novel component involved in removal of 5mC from a set of genes manifesting memory of parent of origin in endosperm in Arabidopsis (maternal allele becomes active via 5mC removal while paternal allele remains methylated and silent). DRE2 is a highly conserved and essential protein in eukaryotes, best known for its role in biogenesis of iron sulfur cluster cofactors in the cytosol.
Here we obtained the first viable dre2 mutant in plants and characterized novel mutant phenotypes both in the reproductive and vegetative phases. The availability of viable dre2 mutants will help uncover new processes with cellular memory dependent on iron containing factors all the way to uncovering underlying molecular mechanisms.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Capturing environmental memory in DNA, with a little help from chromatin2017

    • Author(s)
      Diana Mihaela Buzas
    • Journal Title

      plant and Cell Physiology

      Volume: 10 Issue: 8 Pages: 1302-1312

    • DOI

      10.1093/pcp/pcx092

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Emerging links between iron-sulfur clusters and 5-methylcytosine base excision repair in plants2016

    • Author(s)
      Buzas D M
    • Journal Title

      Genes & Genetic Systems

      Volume: 91 Issue: 2 Pages: 51-62

    • DOI

      10.1266/ggs.16-00015

    • NAID

      130005421223

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] From the laboratory to the field: assaying histone methylation at <i>FLOWERING LOCUS C</i> in naturally growing <i>Arabidopsis halleri</i>2016

    • Author(s)
      Nishio H, Buzas DM, Nagano AJ, Suzuki Y, Sugano S, Ito M, Morinaga S, Kudoh H.
    • Journal Title

      Genes & Genetic Systems

      Volume: 91 Issue: 1 Pages: 15-26

    • DOI

      10.1266/ggs.15-00071

    • NAID

      130005165463

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] From the laboratory to the field: assaying histone methylation at FLOWERING LOCUS C in naturally growing Arabidopsis halleri.2016

    • Author(s)
      Nishio H, Buzas DM, Nagano AJ, Suzuki Y, Sugano S, Ito M, Morinaga SI, Kudoh H.
    • Journal Title

      Genes and Genetics Systems

      Volume: in press

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Uncovering biological roles of DRE2 asa cytosolic component and beyond2016

    • Author(s)
      Diana Buzas
    • Organizer
      Latest Advances in Plant Development & Environmental Response, CSHA
    • Place of Presentation
      AWAJI YUMEBUTAI INTERNATIONAL CENFERENCE CENTER, AWAJI, Hyogo
    • Year and Date
      2016-11-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Epigenetic role for the conserved Fe-S cluster biogenesis protein atDRE2 in Arabidopsis thaliana2015

    • Author(s)
      Diana Buzas
    • Organizer
      Epigenetic Dynamics: Roles in Development, Inheritance and responses to Environment
    • Place of Presentation
      Bentley Univestity, Waltham MA US
    • Year and Date
      2015-08-02
    • Related Report
      2015 Research-status Report
  • [Presentation] Requirements for Iron-Sulfur clusters cofactors in DNA demethylation2015

    • Author(s)
      Diana Buzas
    • Organizer
      A consortium of plant epigeneticsts in Japan- first meeting
    • Place of Presentation
      NIG Mishima, Shizuoka, Japan
    • Year and Date
      2015-07-29
    • Related Report
      2015 Research-status Report
    • Invited

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Published: 2015-04-16   Modified: 2020-03-17  

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