Why and how terminally differentiated cells are kept quiescent

• Project/Area Number: 15K18564
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ikeuchi Momoko 国立研究開発法人理化学研究所, 環境資源科学研究センター, 基礎科学特別研究員 (00633570)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 細胞分裂 / 細胞分化 / 再生 / 細胞リプログラミング / 細胞分化の維持機構 / エピジェネティクス

Outline of Final Research Achievements

Plant cells display remarkable plasticity, yet the potential needs to be controlled in the context of multicellular organisms. We have revealed that a histone modifier PRC2 plays key roles in the maintenance of cellular differentiation status. In this study, I further studied how PRC2 prevents proliferation of terminally differentiated cells. I also found an example of mitotic re-entry and cellular de-differentiation of fully differentiated cells, when plants are exposed to wounding stress.

Research Products

• [Int'l Joint Research] CNRS(フランス)
• [Journal Article] WIND1 promotes shoot regeneration through transcriptional activation of ESR1 in Arabidopsis. (2017)
  • Author(s): 1.Iwase A, Harashima H, Ikeuchi M, Rymen B, Ohnuma M, Komaki S, Morohashi K, Kurata T, Nakata M, Ohme-Takagi M, Grotewold E, Sugimoto K
  • Journal Title: Plant Cell Volume: 1 Issue: 1 Pages: 54-69
  • DOI: 10.1105/tpc.16.00623
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Plant regeneration: cellular origins and molecular mechanisms (2016)
  • Author(s): Momoko Ikeuchi, Yoichi Ogawa, Akira Iwase, Keiko Sugimoto
  • Journal Title: Development Volume: 143 Issue: 9 Pages: 1442-1451
  • DOI: 10.1242/dev.134668
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 植物の再生現象における分化全能性制御の分子機構 (2016)
  • Author(s): 岩瀬哲, 池内桃子, 杉本慶子
  • Journal Title: BSJ review Volume: 7 Pages: 161-176
  • Peer Reviewed / Open Access
• [Journal Article] PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis. (2015)
  • Author(s): Ikeuchi M, Iwase A, Rymen B, Harashima H, Shibata M, Ohnuma M, Breuer C, Morao AK, de Lucas M, De Veylder L., Goodrich J, Brady SM, Roudier F, Sugimoto K.
  • Journal Title: Nature Plants Volume: 1 Issue: 7 Pages: 1-7
  • DOI: 10.1038/nplants.2015.89
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Control of plant cell differentiation by histone modification and DNA methylation (2015)
  • Author(s): Ikeuchi M, Iwase A, Sugimoto K
  • Journal Title: Current Opinioin in Plant Biology Volume: 28 Pages: 60-67
  • DOI: 10.1016/j.pbi.2015.09.004
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] WIND1-based acquisition of regeneration competency in Arabidopsis and rapeseed (2015)
  • Author(s): Iwase A,Mita K,Nonaka S, Ikeuchi M, Koizuka C, Ohnuma M, Ezura H, Imamura J, Sugimoto K
  • Journal Title: Journal of Plant Research Volume: 128 Issue: 3 Pages: 389-397
  • DOI: 10.1007/s10265-015-0714-y
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Wound-induced cellular reprogramming in plants (2017)
  • Author(s): Ikeuchi M
  • Organizer: ITbM seminar
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2017-03-24
  • Invited
• [Presentation] Identification of novel regulator required for wound-induced cellular reprogramming in Arabidopsis thaliana (2017)
  • Author(s): Ikeuchi M, Iwase A, Sugimoto K
  • Organizer: 日本植物生理学会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] Transcriptome and hormonal analysis of wound-induced callus formation in Arabidopsis (2016)
  • Author(s): Ikeuchi M, Iwase A, Kojima M, Sakakibara H, Sugimoto K
  • Organizer: Cold Spring Harbor Asia
  • Place of Presentation: 淡路夢舞台（兵庫県神戸市）
  • Year and Date: 2016-12-01
  • Int'l Joint Research
• [Presentation] 分化を完了した植物細胞の脱分化と細胞分裂再開 (2016)
  • Author(s): 池内桃子
  • Organizer: 基生研研究会「細胞分化を誘導する細胞周期制御システム」
  • Place of Presentation: 基礎生物学研究所（愛知県岡崎市）
  • Year and Date: 2016-11-21
  • Invited
• [Presentation] Stress induced cellular reprogramming in plants (2016)
  • Author(s): Ikeuchi M
  • Organizer: 新学術領域環境記憶統合若手の会
  • Place of Presentation: KKR熱海（静岡県熱海市）
  • Year and Date: 2016-10-12
• [Presentation] Epigenetic control of plant regeneration and stem cell formation (2016)
  • Author(s): Ikeuchi M, Iwase A, Sugimoto K
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学（岩手・盛岡）
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] 植物細胞の分化可塑性およびその抑制機構 (2015)
  • Author(s): 池内桃子
  • Organizer: 日本植物学会
  • Place of Presentation: 朱鷺メッセ（新潟・新潟）
  • Year and Date: 2015-09-06
  • Invited
• [Presentation] PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis (2015)
  • Author(s): Ikeuchi M, Iwase A, Sugimoto K
  • Organizer: FASEB meeting: Mechanisms of Plant Development
  • Place of Presentation: Vermont, USA
  • Year and Date: 2015-08-02
  • Int'l Joint Research
• [Remarks] 植物が傷口で茎葉を再生させる仕組み
  • URL: http://www.riken.jp/pr/press/2017/20170117_2/