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Dedifferentiation to redifferentiation: Molecular mechanism on de novo shoot formation at wound site

Research Project

Project/Area Number 15K18565
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

IWASE AKIRA  国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (40553764)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords細胞リプログラミング / 脱分化 / 再分化 / カルス形成 / 転写因子 / 傷害応答 / ストレス / 分化全能性 / 組織培養 / ナタネ / カルス / 再生 / シロイヌナズナ
Outline of Final Research Achievements

Callus formation and successive de novo organogenesis are seen in nature and in in vitro; however, the molecular mechanisms underlying this control remain poorly understood. In this study, we investigated how wound induced-transcription factors promote callus formation and shoot regeneration at wound site of Arabidopsis.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015 Other

All Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 4 results) Presentation (11 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Simultaneous but spatially different regulation of non-photosynthetic callus formation and photosynthetic root development after shoot removal2017

    • Author(s)
      Kobayashi Koichi、Iwase Akira
    • Journal Title

      Plant Signaling & Behavior

      Volume: 12 Issue: 6 Pages: e1338999-e1338999

    • DOI

      10.1080/15592324.2017.1338999

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] WIND1 promotes shoot regeneration through transcriptional activation of ESR1 in Arabidopsis.2017

    • Author(s)
      1.Iwase A, Harashima H, Ikeuchi M, Rymen B, Ohnuma M, Komaki S, Morohashi K, Kurata T, Nakata M, Ohme-Takagi M, Grotewold E, Sugimoto K
    • Journal Title

      Plant Cell

      Volume: 1 Issue: 1 Pages: 54-69

    • DOI

      10.1105/tpc.16.00623

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Plant regeneration: cellular origins and molecular mechanisms2016

    • Author(s)
      Momoko Ikeuchi, Yoichi Ogawa, Akira Iwase, Keiko Sugimoto
    • Journal Title

      Development

      Volume: 143 Issue: 9 Pages: 1442-1451

    • DOI

      10.1242/dev.134668

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Control of plant cell differentiation by histone modification and DNA methylation2015

    • Author(s)
      Ikeuchi M, Iwase A, Sugimoto K
    • Journal Title

      Current Opinioin in Plant Biology

      Volume: 28 Pages: 60-67

    • DOI

      10.1016/j.pbi.2015.09.004

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis.2015

    • Author(s)
      Ikeuchi M, Iwase A, Rymen B, Harashima H, Shibata M, Ohnuma M, Breuer C, Morao AK, de Lucas M, De Veylder L., Goodrich J, Brady SM, Roudier F, Sugimoto K.
    • Journal Title

      Nature Plants

      Volume: 1 Issue: 7 Pages: 1-7

    • DOI

      10.1038/nplants.2015.89

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] WIND1-based acquisition of regeneration competency in Arabidopsis and rapeseed2015

    • Author(s)
      Iwase A,Mita K,Nonaka S, Ikeuchi M, Koizuka C, Ohnuma M, Ezura H, Imamura J, Sugimoto K
    • Journal Title

      Journal of Plant Research

      Volume: 128 Issue: 3 Pages: 389-397

    • DOI

      10.1007/s10265-015-0714-y

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Wound-induced cellular reprogramming in Arabidopsis2018

    • Author(s)
      Akira Iwase, Bart Rymen, Momoko Ikeuchi, Ayako Kawamura, Takamasa Suzuki, Nobutaka Mitsuda and Keiko Sugimoto
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] WIND1-induced cellular reprogramming in Arabidopsis2016

    • Author(s)
      Akira Iwase, Nobutaka Mitsuda, Kengo Morohashi, Takamasa Suzuki, Hirofumi Harashima, Momoko Ikeuchi, Bart Rymen, Erich Grotewold and Keiko Sugimoto
    • Organizer
      Cold Spring Harbor Asia, Latest Advances in Plant Development & Environmental Response
    • Place of Presentation
      淡路夢舞台国際会議場 (淡路市, 兵庫県)
    • Year and Date
      2016-12-01
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ストレスで誘起される細胞リプログラミングの分子機構2016

    • Author(s)
      岩瀬 哲
    • Organizer
      日本植物学会第80回大会シンポジウム Induced Development:環境要因に誘発される発生の多様性と共通性
    • Place of Presentation
      沖縄コンベンションセンター (宜野湾市, 沖縄県)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 植物の脱分化・再分化にかかわる分子(転写因子と代謝産物)2016

    • Author(s)
      Akira Iwase
    • Organizer
      新学術領域研究「植物部発生ロジックの多元的開拓」第3回メタボローム勉強会
    • Place of Presentation
      鶴岡メタボロームキャンパス(山形県・鶴岡市)
    • Year and Date
      2016-02-13
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Epigenetic control of plant cell dedifferentiation and redifferentiation2015

    • Author(s)
      Akira Iwase, Momoko Ikeuchi, Keiko Sugimoto
    • Organizer
      CRESTシンポジウム「Towards Increased Plant Productivity through Understanding of Environmental Responses and Epigenetic Regulation」
    • Place of Presentation
      理化学研究所 (神奈川県・横浜市)
    • Year and Date
      2015-11-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Shoot regenration pathway upon stresses2015

    • Author(s)
      Akira Iwase, Hirofumi Harashima, Momoko Ikeuchi, Keiko Sugimoto
    • Organizer
      新学術領域研究「植物野成長可塑性を支える環境認識と記憶の自立分散型統御システム」第1回若手の会
    • Place of Presentation
      ホテルウェルシーズン浜名湖 (静岡県・浜松市)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] WIND1-derived redifferentiation pathway in Arabidopsis2015

    • Author(s)
      Akira Iwase, Hirofumi Harashima,Momoko Ikeuchi, Keiko Sugimoto
    • Organizer
      11th International Congress of Plant Molecular Biology(South America)
    • Place of Presentation
      イグアス(ブラジル)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 分化した植物細胞はどこまで可塑性を発揮できるのか?2015

    • Author(s)
      岩瀬哲、池内桃子、杉本慶子
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-08
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] WINDs mediated-plant cell dedifferentiation pathway in Arabidopsis2015

    • Author(s)
      Akira Iwase, Momoko Ikeuchi, Keiko Sugimoto
    • Organizer
      International ERATO Higashiyama Live-Holonics Symposium and Technical Workshop 2015
    • Place of Presentation
      名古屋大学(愛知県・名古屋市)
    • Year and Date
      2015-08-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Epigenetic control of plant cell dedifferentiation under environmental stresses2015

    • Author(s)
      Akira Iwase, Momoko Ikeuchi, Keiko Sugimoto
    • Organizer
      Cold Spring Harbor Asia Conferences, Frontiers of Plant Biology: Epigenetics & Development
    • Place of Presentation
      蘇州(中国)
    • Year and Date
      2015-06-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Epigenetic control of plant cell dedifferentiation under environmental stresses2015

    • Author(s)
      Akira Iwase, Momoko Ikeuchi, Keiko Sugimoto
    • Organizer
      第9回日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター (東京都・千代田区)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] 植物学の百科事典 (項目:傷害応答)2016

    • Author(s)
      岩瀬哲、杉本慶子
    • Total Pages
      802
    • Publisher
      丸善
    • Related Report
      2016 Research-status Report
  • [Book] 植物学の百科事典 (共著:傷害応答担当)2016

    • Author(s)
      岩瀬哲 杉本慶子
    • Publisher
      丸善
    • Related Report
      2015 Research-status Report
  • [Book] 植物の再生現象における分化全能性制御の分子機構 (BSJ review)2016

    • Author(s)
      岩瀬哲 池内桃子 杉本慶子
    • Publisher
      日本植物学会
    • Related Report
      2015 Research-status Report
  • [Remarks] 植物が傷口で茎葉を再生させる仕組み -組織培養による植物の量産や有用物質生産に期待-

    • URL

      http://www.riken.jp/pr/press/2017/20170117_2/

    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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