Analysis of Germ line stem cells playing pivotal roles in multiple reproductive strategies
Project/Area Number |
15K18567
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Morphology/Structure
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Research Institution | University of Tsukuba |
Principal Investigator |
HAYASHI MAKOTO 筑波大学, 生命領域学際研究センター, 助教 (50714838)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 配偶子幹細胞 / 精原幹細胞 / ニジマス / 魚類 / マーカー遺伝子 / 網羅的遺伝子発現解析 |
Outline of Final Research Achievements |
In fish, reproductive strategies greatly differ: such as seasonal breeding, semelparity, iteroparity and so on. Germ line stem cells play pivotal roles in these reproductive strategies. In this study, we try to identify the molecular markers to identify spermatogonial stem cells. As a result, we successfully identify three transcripts preferentially expressed in spermatogonial stem cells. These finding will provide the basis for analyzing the germline stem cells in fish.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Germ Cell-Specific Excision of loxP-Flanked Transgenes in Rainbow Trout Oncorhynchus mykiss2016
Author(s)
Katayama, N., Kume, S., Hattori-Ihara, S., Sadaie, S., Hayashi, M., Yoshizaki, G.
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Journal Title
Biology of Reproduction
Volume: 94
Issue: 4
Pages: 1-12
DOI
Related Report
Peer Reviewed
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[Journal Article] Production of germ cell-deficient salmonids by dead end gene knockdown, and their use as recipients for germ cell transplantation2016
Author(s)
Yoshizaki, G., Takashiba, K., Shimamori, S., Fujinuma, K., Shikina, S., Okutsu, T., Kume, S., Hayashi, M.
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Journal Title
Molecular Reproduction and Development
Volume: 83
Issue: 4
Pages: 298-311
DOI
Related Report
Peer Reviewed
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[Presentation] Identification of the molecular markers preferentially expressed in spermatogonial stem cells in fish2017
Author(s)
Hayashi, M., Iwasaki, Y., Hayashi, T., Ebisawa, M., Sasagawa, Y., Yoshimura, M., Ichida, K., Nikaido, I., Yoshizaki, G.
Organizer
4th World Congress of Reproductive Biology
Related Report
Int'l Joint Research
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[Presentation] Identification of the molecular markers preferentially expressed in spermatogonial stem cells in fish2017
Author(s)
Hayashi, M., Iwasaki, Y., Hayashi, T., Ebisawa, M., Sasagawa, Y., Yoshimura, M., Ichida, K., Nikaido, I., Yoshizaki, G.
Organizer
The International Research Symposium on Regulation of Germ Cell Development in vivo and in vitro
Related Report
Int'l Joint Research
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