Analysis of Germ line stem cells playing pivotal roles in multiple reproductive strategies

• Project/Area Number: 15K18567
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: University of Tsukuba
• Principal Investigator: HAYASHI MAKOTO 筑波大学, 生命領域学際研究センター, 助教 (50714838)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 配偶子幹細胞 / 精原幹細胞 / ニジマス / 魚類 / マーカー遺伝子 / 網羅的遺伝子発現解析

Outline of Final Research Achievements

In fish, reproductive strategies greatly differ: such as seasonal breeding, semelparity, iteroparity and so on. Germ line stem cells play pivotal roles in these reproductive strategies. In this study, we try to identify the molecular markers to identify spermatogonial stem cells. As a result, we successfully identify three transcripts preferentially expressed in spermatogonial stem cells. These finding will provide the basis for analyzing the germline stem cells in fish.

Research Products

• [Journal Article] Production of functional sperm by subcutaneous auto-grafting of immature testes in rainbow trout (2018)
  • Author(s): Hayashi Makoto、Sakuma Daika、Yoshizaki Goro
  • Journal Title: Molecular Reproduction and Development Volume: 85 Issue: 2 Pages: 155-162
  • DOI: 10.1002/mrd.22949
  • Peer Reviewed
• [Journal Article] Germ Cell-Specific Excision of loxP-Flanked Transgenes in Rainbow Trout Oncorhynchus mykiss (2016)
  • Author(s): Katayama, N., Kume, S., Hattori-Ihara, S., Sadaie, S., Hayashi, M., Yoshizaki, G.
  • Journal Title: Biology of Reproduction Volume: 94 Issue: 4 Pages: 1-12
  • DOI: 10.1095/biolreprod.115.136929
  • Peer Reviewed
• [Journal Article] Production of germ cell-deficient salmonids by dead end gene knockdown, and their use as recipients for germ cell transplantation (2016)
  • Author(s): Yoshizaki, G., Takashiba, K., Shimamori, S., Fujinuma, K., Shikina, S., Okutsu, T., Kume, S., Hayashi, M.
  • Journal Title: Molecular Reproduction and Development Volume: 83 Issue: 4 Pages: 298-311
  • DOI: 10.1002/mrd.22625
  • Peer Reviewed
• [Presentation] Identification of the molecular markers preferentially expressed in spermatogonial stem cells in fish (2017)
  • Author(s): Hayashi, M., Iwasaki, Y., Hayashi, T., Ebisawa, M., Sasagawa, Y., Yoshimura, M., Ichida, K., Nikaido, I., Yoshizaki, G.
  • Organizer: 4th World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] Identification of the molecular markers preferentially expressed in spermatogonial stem cells in fish (2017)
  • Author(s): Hayashi, M., Iwasaki, Y., Hayashi, T., Ebisawa, M., Sasagawa, Y., Yoshimura, M., Ichida, K., Nikaido, I., Yoshizaki, G.
  • Organizer: The International Research Symposium on Regulation of Germ Cell Development in vivo and in vitro
  • Int'l Joint Research
• [Presentation] 1細胞RNAseqを用いた魚類精原幹細胞マーカーの探索 (2016)
  • Author(s): 林誠, 岩崎佳子, 海老澤昌史, 芳村美佳, 林 哲太郎, 笹川洋平, 二階堂 愛, 吉崎悟朗
  • Organizer: 平成28年度 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス（東京都港区）
  • Year and Date: 2016-03-28
• [Patent(Industrial Property Rights)] 宿主生殖腺への生着能を有する生殖細胞識別マーカー (2016)
  • Inventor(s): 林誠, 二階堂愛, 吉崎悟朗（他5名）
  • Industrial Property Rights Holder: 国立大学法人 東京海洋大学、国立大学法人 筑波大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-060914
  • Filing Date: 2016-03-24