| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this study, we performed transcriptomes of mouse ovarian theca/ interstitial cells by RNA-seq. Gene ontology analysis demonstrated that the theca/ interstitial cell-specific genes were largely categorized into four major groups. the specific and unique expression of newly detected representative genes, including nidogen 1 Spondin 1, and Aspn/ PLAP1, in theca cells and the adjacent interstitial cells of secondary, preantral and antral follicles were also confirmed. Particularly, expression of Aspn/PLAP1 was detected specifically in outer theca cells. Intriguingly, an Aspn/ PLAP1 antibody completely arrested the growth of secondary follicles which is gonadotropin-independent follicle developmental stage. Altogether, these results suggest that theca/ interstitial cells are classified into subpopulations on the basis of their functions, and provide evidence that Aspn/ PLAP1 is expressed exclusively in outer theca cells and plays a pivotal role in the growth of secondary follicles.
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