| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
In previous studies, anti-mullerian hormone (amh) signal deficient medaka hotei were isolated and they showed hyperactivation of germline stem cell division, resulting germ cell excess. I performed medaka ovarian cell culture and examined the character of cultured cells . After 14 days of cell culture, functional sperm were produced from cultured ovarian germ cells, suggesting that medaka adult ovaries have germ cells with potential to differentiate into both oocytes and sperm.
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