| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
I first analyzed the basic physiological properties of microglia in vitro by combining electrophysiology, calcium imaging, and membrane potential imaging. Specifically, we found that voltage-gated proton channel, one of voltage-gated ion channels of microglia, is important for the regulation of reactive oxigen species (ROS) production. It also contributed to infarct volume in brain ischemia in age-dependent manner.
Next, in order to analyze the importance of this phenomenon in vivo, we started the experiment using zebrafish, which is an excellent animal modle for in vivo study. I used CRISPR/Cas9 system and succeeded in establishing Voltage-gated proton channel deficient zebrafsih. Furthermore, we succeeded in establishing the ROS measurement system in zebrafish, allowing us to perform further detailed analysis in near future.
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