Neuromodulatory funstion of microglia in neural circuits
| Project/Area Number |
15K18575
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Animal physiology/Animal behavior
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
|---|
| Keywords | ミクログリア / 電位依存性プロトンチャネル / CRISPR/Cas9 / ゼブラフィッシュ |
|---|
| Outline of Final Research Achievements |
I first analyzed the basic physiological properties of microglia in vitro by combining electrophysiology, calcium imaging, and membrane potential imaging. Specifically, we found that voltage-gated proton channel, one of voltage-gated ion channels of microglia, is important for the regulation of reactive oxigen species (ROS) production. It also contributed to infarct volume in brain ischemia in age-dependent manner.
Next, in order to analyze the importance of this phenomenon in vivo, we started the experiment using zebrafish, which is an excellent animal modle for in vivo study. I used CRISPR/Cas9 system and succeeded in establishing Voltage-gated proton channel deficient zebrafsih. Furthermore, we succeeded in establishing the ROS measurement system in zebrafish, allowing us to perform further detailed analysis in near future.
|
Report
(3 results)
Research Products
(5 results)
