| Project/Area Number |
15K18579
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Konan University |
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Principal Investigator |
Ohta Akane 甲南大学, 自然科学研究科, 研究員 (50410717)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | C. elegans / 3量体Gタンパク質 / 線虫 / カルシウムイメージング / 温度受容 / 低温耐性 / GPCR / 温度情報伝達 / 分子神経遺伝学 / 細胞遺伝 |
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| Outline of Final Research Achievements |
Temperature is important information for biological reaction. Animals have acclimation mechanism to temperature changes. C. elegans has cultivation temperature-dependent cold tolerance. We are using the cold tolerance as a model for studying temperature signaling in animal. Previously reported, ASJ sensory neuron that is known as light and pheromone-sensing neuron senses temperature through trimeric G-protein pathway, which is required for photo signaling. However, photoreceptor LITE-1 was not involved in cold tolerance and temperature sensation in ASJ. In this study, we found that multiple G-proteins additively regulate temperature signaling in ASJ. To identify the temperature sensing receptor upstream of trimeric G protein, we screened G protein-coupled receptor (GPCR). We performed RNAi screening for GPCR genes. We constructed knockout mutants of respective GPCR genes by using CRISPR/Cas9 system, and analyzed cold tolerance.
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