Temperature signaling through trimeric G protein in sensoryneuron of C. elegans
Project/Area Number |
15K18579
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Genetics/Chromosome dynamics
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Research Institution | Konan University |
Principal Investigator |
Ohta Akane 甲南大学, 自然科学研究科, 研究員 (50410717)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | C. elegans / 3量体Gタンパク質 / 線虫 / カルシウムイメージング / 温度受容 / 低温耐性 / GPCR / 温度情報伝達 / 分子神経遺伝学 / 細胞遺伝 |
Outline of Final Research Achievements |
Temperature is important information for biological reaction. Animals have acclimation mechanism to temperature changes. C. elegans has cultivation temperature-dependent cold tolerance. We are using the cold tolerance as a model for studying temperature signaling in animal. Previously reported, ASJ sensory neuron that is known as light and pheromone-sensing neuron senses temperature through trimeric G-protein pathway, which is required for photo signaling. However, photoreceptor LITE-1 was not involved in cold tolerance and temperature sensation in ASJ. In this study, we found that multiple G-proteins additively regulate temperature signaling in ASJ. To identify the temperature sensing receptor upstream of trimeric G protein, we screened G protein-coupled receptor (GPCR). We performed RNAi screening for GPCR genes. We constructed knockout mutants of respective GPCR genes by using CRISPR/Cas9 system, and analyzed cold tolerance.
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Report
(3 results)
Research Products
(31 results)
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[Journal Article] Natural variations of cold tolerance and temperature acclimation in Caenorhabditis elegans2016
Author(s)
Okahata M., Ohta, A., Mizutani, H., Minakuchi, Y., Toyoda, A., Kuhara, A.
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Journal Title
Journal of Comparative Physiology B.
Volume: 186(8)
Issue: 8
Pages: 985-998
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] 線虫の温度適応の制御機構2016
Author(s)
Akane Ohta
Organizer
ICZ22 ZSJ87 jointmeeting 2016
Place of Presentation
沖縄コンベンションセンター(宜野湾、Okinawa)
Year and Date
2016-11-16
Related Report
Int'l Joint Research / Invited
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[Presentation] Tomoyo Ujisawa, Misato Uda, Tatsuya Ii, Miki Ii, Akane Ohta, Atsushi Kuhara2016
Author(s)
Tomoyo Ujisawa, Misato Uda, Tatsuya Ii, Miki Ii, Akane Ohta, Atsushi Kuhara
Organizer
C. elegans Neuronal Development, Synaptic Function & Behavior meeting
Place of Presentation
Nagoya university(Nagoya, Aichi, Japan)
Year and Date
2016-07-27
Related Report
Int'l Joint Research
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