Genetic control of plastid division in chlorarachniophytes

• Project/Area Number: 15K18582
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: University of Tsukuba
• Principal Investigator: HIRAKAWA Yoshihisa 筑波大学, 生命環境系, 助教 (40647319)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 葉緑体 / オルガネラ / 分裂 / DNA複製 / 藻類 / ヌクレオモルフ / トランスクリプトーム

Outline of Final Research Achievements

I have investigated the mechanism and evolution of organelle division and organelle DNA replication in the secondary plastid-bearing algae, chlorarachniophytes. I newly identified several proteins for constriction of the inner plastid membranes and for replication of plastid, mitochondrial, and nucleomorph DNA. I have discussed the evolution of those proteins based on molecular phylogeny.

Research Products

• [Journal Article] Multiple losses of photosynthesis and convergent reductive genome evolution in the colourless green algae Prototheca (2018)
  • Author(s): Shigekatsu Suzuki, Rikiya Endoh, Ri-ichiroh Manabe, Moriya Ohkuma, Yoshihisa Hirakawa
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 940-940
  • DOI: 10.1038/s41598-017-18378-8
  • Peer Reviewed / Open Access
• [Journal Article] クロララクニオン藻の色素体制御機構 (2017)
  • Author(s): 平川泰久
  • Journal Title: BSJ-Review Volume: 8 Issue: C Pages: 169-174
  • DOI: 10.24480/bsj-review.8c6.00125
  • ISSN: 2432-9819
  • Peer Reviewed / Open Access
• [Journal Article] Evolution of UhpC-type hexose-phosphate transporters in dinoflagellates. (2017)
  • Author(s): Yoshihisa Hirakawa, Takuro Nakayama, Nadine Keilert, Ken-ichiro Ishida
  • Journal Title: Phycological Research Volume: 65 Issue: 2 Pages: 166-170
  • DOI: 10.1111/pre.12164
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Plastid genome sequences of Gymnochlora stellata, Lotharella vacuolata, and Partenskyella glossopodia reveal remarkable structural conservation among chlorarachniophyte species. (2016)
  • Author(s): Suzuki S, Hirakawa Y, Kofuji R, Sugita M, Ishida K.
  • Journal Title: Journal of Plant Research Volume: 印刷中 Issue: 4 Pages: 581-590
  • DOI: 10.1007/s10265-016-0804-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Diurnal Transcriptional Regulation of Endosymbiotically Derived Genes in the Chlorarachniophyte Bigelowiella natans. (2016)
  • Author(s): Shigekatsu Suzuki, Ken-Ichiro Ishida, Yoshihisa Hirakawa
  • Journal Title: Genome Biology and Evolution Volume: 8 Issue: 9 Pages: 2672-2682
  • DOI: 10.1093/gbe/evw188
  • NAID: 120007129385
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prospective function of FtsZ proteins in the secondary plastid of chlorarachniophyte algae (2015)
  • Author(s): Yoshihisa Hirakawa and Ken-ichiro Ishida
  • Journal Title: BMC Plant Biology Volume: 15 Issue: 1 Pages: 276-276
  • DOI: 10.1186/s12870-015-0662-7
  • NAID: 120007135803
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Complex plastid evolution in chlorarachniophyte algae (2017)
  • Author(s): Yoshihisa Hirakawa
  • Organizer: Company of Biologists Workshop "Symbiosis in the microbial world: from ecology to genome evolution"
  • Int'l Joint Research / Invited
• [Presentation] クロララクニオン藻のダイナミン様タンパク質 (2017)
  • Author(s): 平川泰久、石田 健一郎
  • Organizer: 日本植物学会
• [Presentation] クロララクニオン藻の遺伝子発現から紐解く色素体制御機構 (2016)
  • Author(s): 平川泰久、鈴木 重勝、石田 健一郎
  • Organizer: 日本植物学会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-09-16
• [Presentation] クロララクニオン藻のオルガネラDNA ポリメラーゼの進化 (2016)
  • Author(s): 渡辺ありさ、鈴木重勝、平川泰久、石田 健一郎
  • Organizer: 日本植物学会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-09-16
• [Presentation] Evolution of organellar DNA polymerases in Chlorarachniophyte algae. (2016)
  • Author(s): Arisa Watanabe, Shigekatsu Suzuki, Yoshihisa Hirakawa, Ken-ichiro Ishida
  • Organizer: ICES 2016
  • Place of Presentation: 京都府立大学（ 京都市左京区）
  • Year and Date: 2016-09-10
  • Int'l Joint Research
• [Presentation] Organelle DNA replication in chlorarachniophyte algae. (2016)
  • Author(s): Yoshihisa Hirakawa, Arisa Watanabe, Shigekatsu Suzuki, Ken-ichiro Ishida
  • Organizer: Protist 2016
  • Place of Presentation: モスクワ大学（ロシア・モスクワ）
  • Year and Date: 2016-06-06
  • Int'l Joint Research
• [Presentation] クロララクニオン藻における日周期による遺伝子発現プロファイル (2016)
  • Author(s): 鈴木 重勝、石田 健一郎、平川 泰久
  • Organizer: 日本藻類学会
  • Place of Presentation: 日本歯科大学（東京都千代田区）
  • Year and Date: 2016-03-19
• [Presentation] クロララクニオン藻の細胞周期における遺伝子発現プロファイル (2015)
  • Author(s): 平川泰久
  • Organizer: 日本植物学会
  • Place of Presentation: 新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2015-09-06
• [Book] Secondary endosymbioses (2017)
  • Author(s): Yoshihisa Hirakawa
  • Total Pages: 452
  • Publisher: Academic press
  • ISBN: 9780128026519
• [Remarks] 平川泰久ホームページ
  • URL: http://yhirakawa.weebly.com