How do evolutionary novelties emerge? An approach with genome editing
Project/Area Number |
15K18586
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Evolutionary biology
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Research Institution | Kyoto University |
Principal Investigator |
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Research Collaborator |
FUKUTOMI Yuichi 京都大学, 大学院理学研究科, 大学院生
Matsumoto KEIJI 京都大学, 大学院理学研究科, 研究員
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 進化発生学 / ショウジョウバエ / 模様 / ゲノム編集 |
Outline of Final Research Achievements |
We attempted to introduce genome editing technique by CRISPR / Cas9 system to Drosophila guttifera, aiming to clarify the role of genes in color pattern formation and the regulatory relationship among genes. We first established a method to quantitatively measure development of pigmentation in wings by image analysis. Next, with reference to the method used in Drosophila melanogaster CRISPR / Cas 9 system, genes on the X chromosome were targeted to show the effectiveness of the method. As a result of trial and error, we established a method to make G0 mosaic individuals and knockout strains with mutation in the germline.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.2016
Author(s)
Hashimoto T, Horikawa DD, Saito Y, Kuwahara H, Kozuka-Hata H, Shin-I T, Minakuchi Y, Ohishi K, Motoyama A, Aizu T, Enomoto A, Kondo K, Tanaka S, Hara Y, Koshikawa S, Sagara H, Miura T, Yokobori S, Miyagawa K, Suzuki Y, Kubo T, Oyama M, Kohara Y, Fujiyama A, Arakawa K, Katayama T, Toyoda A and Kunieda T
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Journal Title
Nature Communications
Volume: 7
Issue: 1
Pages: 12808-12808
DOI
NAID
Related Report
Peer Reviewed / Open Access
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