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How do evolutionary novelties emerge? An approach with genome editing

Research Project

Project/Area Number 15K18586
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Research InstitutionKyoto University

Principal Investigator

Koshikawa Shigeyuki  京都大学, 白眉センター, 特定助教 (30714498)

Research Collaborator FUKUTOMI Yuichi  京都大学, 大学院理学研究科, 大学院生
Matsumoto KEIJI  京都大学, 大学院理学研究科, 研究員
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords進化発生学 / ショウジョウバエ / 模様 / ゲノム編集
Outline of Final Research Achievements

We attempted to introduce genome editing technique by CRISPR / Cas9 system to Drosophila guttifera, aiming to clarify the role of genes in color pattern formation and the regulatory relationship among genes.
We first established a method to quantitatively measure development of pigmentation in wings by image analysis. Next, with reference to the method used in Drosophila melanogaster CRISPR / Cas 9 system, genes on the X chromosome were targeted to show the effectiveness of the method. As a result of trial and error, we established a method to make G0 mosaic individuals and knockout strains with mutation in the germline.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (19 results)

All 2017 2016 2015

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (2 results)

  • [Journal Article] Pupal development and pigmentation process of a polka-dotted fruit fly, Drosophila guttifera (Insecta, Diptera)2017

    • Author(s)
      Fukutomi Y, Matsumoto K, Agata K, Funayama N, Koshikawa S
    • Journal Title

      Development Genes and Evolution

      Volume: 227 Issue: 3 Pages: 171-180

    • DOI

      10.1007/s00427-017-0578-3

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.2016

    • Author(s)
      Hashimoto T, Horikawa DD, Saito Y, Kuwahara H, Kozuka-Hata H, Shin-I T, Minakuchi Y, Ohishi K, Motoyama A, Aizu T, Enomoto A, Kondo K, Tanaka S, Hara Y, Koshikawa S, Sagara H, Miura T, Yokobori S, Miyagawa K, Suzuki Y, Kubo T, Oyama M, Kohara Y, Fujiyama A, Arakawa K, Katayama T, Toyoda A and Kunieda T
    • Journal Title

      Nature Communications

      Volume: 7 Issue: 1 Pages: 12808-12808

    • DOI

      10.1038/ncomms12808

    • NAID

      120005971035

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phylogeography of the subgenus Drosophila (Diptera: Drosophilidae): Evolutionary history of faunal divergence between the Old and the New Worlds2016

    • Author(s)
      Izumitani HF, Kusaka Y, Koshikawa S, Toda MJ, Katoh T
    • Journal Title

      PLoS ONE

      Volume: 11(7) Issue: 7 Pages: e0160051-e0160051

    • DOI

      10.1371/journal.pone.0160051

    • NAID

      120005947177

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gain of cis-regulatory activities underlies novel domains of wingless gene expression in Drosophila2015

    • Author(s)
      Shigeyuki Koshikawa, Matt W Giorgianni, Kathy Vaccaro, Victoria A Kassner, John H Yoder, Thomas Werner, Sean B Carroll
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 112 Issue: 24 Pages: 7524-7529

    • DOI

      10.1073/pnas.1509022112

    • NAID

      120005611429

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Enhancer Modularity and the Evolution of New Traits2015

    • Author(s)
      Shigeyuki Koshikawa
    • Journal Title

      Fly (Austin)

      Volume: 9 Issue: 4 Pages: 155-159

    • DOI

      10.1080/19336934.2016.1151130

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Wing color pattern formation in a polka-dotted fruit fly Drosophila guttifera2017

    • Author(s)
      越川滋行、福冨雄一、松本圭司
    • Organizer
      第22回国際動物学会/第87回日本動物学会合同大会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2017-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Unique mechanism of pigmentation pattern formation in the polka-dotted fly, Drosophila guttifera2017

    • Author(s)
      福冨雄一、船山典子、越川滋行
    • Organizer
      第22回国際動物学会/第87回日本動物学会合同大会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2017-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ミズタマショウジョウバエの模様形成の仕組み2017

    • Author(s)
      越川滋行
    • Organizer
      第87回日本蚕糸学会
    • Place of Presentation
      農林水産技術会議事務局 筑波産学連携支援センター
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ミズタマショウジョウバエの水玉模様には意味があるのか?-模様の生態における機能を探る-2017

    • Author(s)
      越川滋行、松本圭司、福冨雄一
    • Organizer
      第64回日本生態学会
    • Place of Presentation
      早稲田大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] ミズタマショウジョウバエの蛹期の翅の発生様式と斑紋形成プロセス2016

    • Author(s)
      福冨雄一、阿形清和、越川滋行
    • Organizer
      日本昆虫学会第76回大会・第60回日本応用動物昆虫学会大会合同大会
    • Place of Presentation
      大阪府立大学(大阪府・堺市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] ミズタマショウジョウバエの模様形成機構の理解に向けて2016

    • Author(s)
      越川滋行、福冨雄一、松本圭司
    • Organizer
      日本遺伝学会第88回大会
    • Place of Presentation
      日本大学三島駅北口校舎
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Wing development and pigmentation process in pupae of polka-dotted fly Drosophila guttifera2016

    • Author(s)
      福冨雄一、船山典子、阿形清和、越川滋行
    • Organizer
      JSDB Special Symposium: Frontier of Developmental Biology (+ 49th Meeting)
    • Place of Presentation
      東京大学本郷キャンパス
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ミズタマショウジョウバエの水玉模様の形成と進化2015

    • Author(s)
      越川滋行
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(兵庫県・神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Research-status Report
  • [Presentation] Drosophila guttiferaにおける水玉模様形成とその進化2015

    • Author(s)
      越川滋行
    • Organizer
      ショウジョウバエ多様性研究会
    • Place of Presentation
      国立遺伝学研究所(静岡県・三島市)
    • Year and Date
      2015-09-28
    • Related Report
      2015 Research-status Report
  • [Presentation] ミズタマショウジョウバエの翅の模様を制御する因子2015

    • Author(s)
      越川滋行
    • Organizer
      日本動物学会第86回大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 模様の進化を引き起こした遺伝的変化2015

    • Author(s)
      越川滋行
    • Organizer
      日本進化学会第17回大会
    • Place of Presentation
      中央大学(東京都・文京区)
    • Year and Date
      2015-08-21
    • Related Report
      2015 Research-status Report
  • [Presentation] Toward understanding color pattern formation and evolution in Drosophila2015

    • Author(s)
      Koshikawa S, Giorgianni MW, Vaccaro K, Kassner VA, Yoder JH, Werner T, Carroll SB
    • Organizer
      日本発生生物学会第48回大会
    • Place of Presentation
      つくば国際会議場(茨城県・つくば市)
    • Year and Date
      2015-06-03
    • Related Report
      2015 Research-status Report
  • [Book] Diversity and evolution of butterfly wing patterns: an integrative approach2017

    • Author(s)
      Koshikawa S, Fukutomi Y, Matsumoto K (分担)
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report
  • [Book] チョウの斑紋多様性と進化 - 統合的アプローチ2017

    • Author(s)
      越川滋行、福冨雄一、松本圭司 (分担)
    • Publisher
      海游舎
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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