| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
PSMB8 is one of the proteasome beta subunits with protease activity responsible for generating MHC class I presenting-peptides. PSMB8 gene of cartilaginous and bony fish has two (A and F) lineages showing about 20% differences of amino acid residues. In bony fish, two lineages are presented as dimorphic alleles maintaining for about 400 million years among species. To clarify the biological factors maintaining the dimorphic alleles, cleavage specificities were compared between A and F alleles. Whereas immunoproteasome containing A lineage of PSMB8 increased chymotrypsin-like activity compared with constitutive proteasome, it having F lineage of PSMB8 decreased. this result implied that two lineages of PSMB8 supply the MHC class I presenting-pepties with various C-terminal residues.
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