| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
To understand molecular mechanism regulating plant leaf shape variation, this study conducted leaf anatomy of narrow leaves in rheophytic plants which grow in riverbanks. The development of narrow leaf shape was found to be mainly caused by active cell division and proliferation. Immunohistochemical analysis suggested that auxin accumulation in the top and base of the leaf blade could promote activation of cell proliferation and development the main vein, leading to the longitudinal elongation of leaf blade. Transcriptome analysis showed that the auxin-related genes were significantly activated in developing leaves. Comparative analysis between rheophytic plants and their closely related species with broad leaves showed that the expression patterns of several genes regulating auxin transport and signaling were significantly different in the young leaves between the narrow and broad leaves. These genes are the candidates that could play important roles in leaf shape variation.
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