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Genomics of intranuclear bacteria of the termite gut protist and gene transfer from the bacteria to the host

Research Project

Project/Area Number 15K18604
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionTokyo Institute of Technology

Principal Investigator

Kuwahara Hirokazu  東京工業大学, 生命理工学院, 助教 (10528053)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords共生 / ゲノム / 核内共生 / 難培養微生物 / シロアリ / 昆虫 / 細胞質共生
Outline of Final Research Achievements

Termites harbor protists and prokaryotes in their guts. Furthermore the protists generally establish a symbiotic relationship with multiple species of prokaryotes, which reside in their cytoplasm, attach onto the cell surface or nucleoplasm. However the functions of the symbionts and the symbiotic mechanisms mostly remain unclear due to difficulties to culture the symbionts and the hosts. In this study, we have performed a genome analysis of intranuclear Verrucomicrobial symbionts of Trichonympha agilis. The genome analysis suggests that the intranuclear symbionts are not able to synthesize many metabolites and depend on the host for the metabolites. In addition, the genomes have partial genes for type III secretion system, suggesting that the type III secretion system is possibly utilized for the host-symbiont interaction. The genome analysis of intranuclear symbionts revealed part of the symbiotic relationship with the host.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 2016

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (3 results)

  • [Journal Article] Phylogenetic Diversity and Single-Cell Genome Analysis of “<i>Melainabacteria</i>”, a Non-Photosynthetic Cyanobacterial Group, in the Termite Gut2018

    • Author(s)
      Utami Yuniar Devi、Kuwahara Hirokazu、Murakami Takumi、Morikawa Takahiro、Sugaya Kaito、Kihara Kumiko、Yuki Masahiro、Lo Nathan、Deevong Pinsurang、Hasin Sasitorn、Boonriam Warin、Inoue Tetsushi、Yamada Akinori、Ohkuma Moriya、Hongoh Yuichi
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 1 Pages: 50-57

    • DOI

      10.1264/jsme2.ME17137

    • NAID

      130006581744

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Discovery of ectosymbiotic Endomicrobium lineages associated with protists in the gut of stolotermitid termites2017

    • Author(s)
      Izawa Kazuki、Kuwahara Hirokazu、Sugaya Kaito、Lo Nathan、Ohkuma Moriya、Hongoh Yuichi
    • Journal Title

      Environmental Microbiology Reports

      Volume: 9 Issue: 4 Pages: 411-418

    • DOI

      10.1111/1758-2229.12549

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Draft Genome Sequence of Lactococcus sp. Strain Rs-Y01, Isolated from the Gut of the Lower Termite Reticulitermes speratus2017

    • Author(s)
      Yuki Masahiro、Sakamoto Mitsuo、Kuwahara Hirokazu、Hongoh Yuichi、Ohkuma Moriya
    • Journal Title

      Genome Announcements

      Volume: 5 Issue: 38

    • DOI

      10.1128/genomea.00999-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome of ‘Ca. Desulfovibrio trichonymphae’, an H2-oxidizing bacterium in a tripartite symbiotic system within a protist cell in the termite gut2017

    • Author(s)
      Kuwahara H., Yuki M., Izawa K., Ohkuma M, Hongoh Y
    • Journal Title

      The ISME Journal

      Volume: 11 Issue: 3 Pages: 766-776

    • DOI

      10.1038/ismej.2016.143

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] シロアリ腸内原生生物の核内に共生する未培養Verrucomicrobia門細菌の多様性とゲノム解析2018

    • Author(s)
      名倉裕一
    • Organizer
      ゲノム微生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Functional genomics of multiple endo- and ectosymbionts of termite gut protists2017

    • Author(s)
      桑原宏和
    • Organizer
      “BIOLOGY OF SYMBIOSIS" WORKSHOP
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロアリ腸内原生生物に共生する Desulfovibrio 属細菌のゲノム解析による3者間共生システムの解明2016

    • Author(s)
      桑原宏和、雪真弘、伊澤和輝、大熊盛也、本郷裕一
    • Organizer
      日本微生物生態学会 第 31 回大会
    • Place of Presentation
      横須賀
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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