The comparative study of adaptation strategy for hydrogen sulfide between deep-sea polychaete species

• Project/Area Number: 15K18616
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Nihon University
• Principal Investigator: KOITO Tomoko 日本大学, 生物資源科学部, 講師 (10583148)
• Research Collaborator: INOUE Koji ; NAGASAKI Toshihiro
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: タウリン合成経路 / ヒポタウリン / チオタウリン / 深海性多毛類 / 硫化水素無毒化 / 硫化水素 / 熱水噴出域

Outline of Final Research Achievements

The deep-sea hydrothermal vents are known to belch out sulfide-rich fluid. Although hydrogen sulfide is harmful for animals, it has been observed the flourishing invertebrate communities. In this study, we focused on the two polychaete species (Paralvinella hessleri and phyllodocida) which inhabit sympatrically, to reveal the adaptation mechanism for ambient hydrogen sulfide. Based on our results, we found that both species synthesize the hypotaurine and taurine in their body using taurine synthesis pathway which was identical with mammalian pathway. Furthermore, the expression levels of enzyme genes which involve in taurine synthesis were up or down regulated by sulfide exposure level. Thus, it is considered that deep-sea polychaetes could response to ambient hydrogen sulfide and change the level of synthesis of taurine.

Research Products

• [Journal Article] Simultaneous analysis of free amino acids and taurine-related compounds in deep-sea mussel tissues using reversed-phase HPLC (2018)
  • Author(s): Toshihiro Nagasaki, Tomoko Koito, Suguru Nemoto, Hideki Ushio, Koji Inoue
  • Journal Title: Fisheries Science Volume: 84 Issue: 1 Pages: 127-134
  • DOI: 10.1007/s12562-017-1143-8
  • NAID: 40021443698
  • Peer Reviewed
• [Journal Article] A molecular framework for the taxonomy and systematics of Japanese marine turbellarian flatworms (Platyhelminthes, Polycladida). (2017)
  • Author(s): Tsunashima, T., Hagiya, M., Yamada, R., Koito, T., Tsuyuki, N., Izawa, S., Kosoba, K., Itoi, S. and Sugita, H
  • Journal Title: Aquatic Biology Volume: 26 Pages: 159-167
  • Peer Reviewed / Open Access
• [Presentation] 熱水噴出域近傍に生息する多毛類の硫化物適応機構 (2017)
  • Author(s): 佐々木学人、小糸智子、井上広滋
  • Organizer: ブルーアース2017
  • Place of Presentation: 日本大学（東京都・千代田区）
  • Year and Date: 2017-03-02
• [Presentation] Utilization of taurine accumulation systems for adaptation to extreme environments in bivalves (2017)
  • Author(s): Koji Inoue, Toshihiro Nagasaki, Azusa Kinjo-Kakumura, Tomoko Koito
  • Organizer: International Symposium "Fisheries Science for Future Generation"
  • Int'l Joint Research
• [Presentation] 深海性・浅海性多毛類のタウリン関連化合物代謝の比較 (2016)
  • Author(s): 小糸智子、齋藤 淑、長﨑稔拓、井上広滋
  • Organizer: 日本水産学会
  • Place of Presentation: 近畿大学（奈良県・奈良市）
  • Year and Date: 2016-09-10