| Project/Area Number |
15K18624
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Hirosaki University |
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Principal Investigator |
Kasai Atsushi 弘前大学, 農学生命科学部, 研究員 (80633982)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | エピゲノム編集 / 新育種技術（NPBT) / 接ぎ木 / 篩管長距離輸送性RNA / 新育種技術NPBT / RNAの篩管長距離輸送性 / GrIGSシステム |
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| Outline of Final Research Achievements |
Novel plant breeding technique named as GrIGS (Graft-induced Gene silencing) system, which fully utilize grafting and phloem long-distance transportability of RNA, has been developed. In this study, I tried to apply the GrIGS system to a vegetative propagation crop potato and to obtain epigenome editing plants.
In model experiment using transgene (35S:GFP), specific method of potato was established by modification of GrIGS system. Then I developed this system to endogenous genes to give additional useful trait (suppression of cold-induced sweetening and degradation of amylose contents) for practical use. At least next generation tubers maintained higher methylation level induced by GrIGS system.
Furthermore, I examined the stack, which was achieved by re-grafting with other siRNA donor to target multiple genes and the high methylation level induction method (the second), with same one to increase the methylation level.
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