| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Petal color lability is a prominent feature of bicolor dahlia cultivars, that causes plants to produce not only original bicolor petals with colored bases and pure white tips, but also frequently single-colored petals without white tips. In this study, we analyzed the molecular mechanisms that are associated with petal color lability using the red-white bicolor cultivar ‘Yuino’. Molecular biological analyses such as qRT-PCR and small RNA mapping analysis, suggest that post-transcriptional gene silencing of DvCHS2 plays a key role in phenotypic lability in this bicolor dahlia. Genomic analysis also suggested that DvCHS2 is the key gene involved in bicolor formation. Identification of flavonoids in leaves also confirms the importance of chalcone synthase for phenotypic lability in ‘Yuino’. And, by using the presence or absence of leaf flavonoid as an indicator, it was suggested that plants producing only original bicolor petals could be selected.
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