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Genetic analysis of Capsicum bio-resource to improve low-pungent capsaicin analogs in chili pepper fruit

Research Project

Project/Area Number 15K18640
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Horticultural science
Research InstitutionOkayama University

Principal Investigator

Tanaka Yoshiyuki  岡山大学, 環境生命科学研究科, 准教授 (20704480)

Research Collaborator YOSHIDA Yuichi  岡山大学, 環境生命科学研究科, 教授 (00141474)
GOTO Tanjuro  岡山大学, 環境生命科学研究科, 教授 (40195938)
YASUBA Kenichiro  岡山大学, 環境生命科学研究科, 准教授 (60343977)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsトウガラシ / カプサイシン / カプシノイド / 辛味成分
Outline of Final Research Achievements

Capsinoids are low-pungent capsaicinoid analogues in chili pepper fruits. They exhibit health-promoting properties in humans similar to capsaicinoids, but do not cause a nasty burning sensation, encouraging their application in foods. The aim of this study was to identify genetic factors to increase capsinoid in Capsicum.
In this study, we identified novel mutant alleles of putative aminotransferase (pAMT) to enhance capsinoid biosynthesis. In addition, we compared an extremely pungent cultivar with other cultivars of different pungency levels. The results indicated that the capsaicinoid biosynthesis in the pericarp contributes to the higher capsaicinoid and its analogs concentrations in fruits, and multiple capsaicinoid biosynthetic genes were strongly expressed exclusively in the pericarp of the extremely pungent cultivar. The results will be useful for pungency and capsinoid breeding.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (13 results)

All 2018 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Identification of a Novel Mutant <i>pAMT</i> Allele Responsible for Low-pungency and Capsinoid Production in Chili Pepper: Accession ‘No. 4034’ (<i>Capsicum chinense</i>)2018

    • Author(s)
      Y.Tanaka, S. Fukuta, S.Koeda, T. Goto, Y. Yoshida, K. Yasuba
    • Journal Title

      The Horticulture Journal

      Volume: 87 Issue: 2 Pages: 222-228

    • DOI

      10.2503/hortj.OKD-115

    • NAID

      130006708534

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Non-pungency in a Japanese Chili Pepper Landrace (<i>Capsicum annuum</i>) is Caused by a Novel Loss-of-function <i>Pun1</i> Allele2017

    • Author(s)
      E. Kirii, T. Goto, Y. Yoshida, K. Yasuba and Y. Tanaka
    • Journal Title

      The Horticulture Journal

      Volume: 86 Issue: 1 Pages: 61-69

    • DOI

      10.2503/hortj.MI-148

    • NAID

      130005312842

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Difference in capsaicinoid biosynthesis gene expression in the pericarp reveals elevation of capsaicinoid contents in chili peppers (Capsicum chinense)2017

    • Author(s)
      Y. Tanaka, F. Nakashima, E. Kirii, T, Goto, Y. Yoshida and K. Yasuba
    • Journal Title

      Plant Cell Reports

      Volume: 36 Issue: 2 Pages: 267-279

    • DOI

      10.1007/s00299-016-2078-8

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] トウガラシ(C. chinense)における異なるpAMTアリルを利用した辛味調整の可能性と転写産物解析2018

    • Author(s)
      淺野高弥・金光世利香・後藤丹十郎・吉田裕一・安場健一郎・田中義行
    • Organizer
      平成30年度園芸学会秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トウガラシ(Capsicum chinense)におけるカプシノイド合成に関わる新規 putative aminotransferase(pAMT)変異アレルの同定2017

    • Author(s)
      田中義行・福多志穂・ 小枝壮太 ・後藤丹十郎・吉田裕一・安場健一郎
    • Organizer
      園芸学会中四国支部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トウガラシ(Capsicum chinense)交雑集団における辛味関連成分に関する QTL pAMT とその効果2017

    • Author(s)
      田中義行・キリーエラスムス・後藤丹十郎・吉田裕一・安場健一郎
    • Organizer
      平成29年度園芸学会秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トウガラシ遺伝資源を用いた辛味成分カプサイシノイドおよびその類似物質に関する成分育種学研究2016

    • Author(s)
      田中義行
    • Organizer
      第8回 中国地域育種談話会・第11回 ムギ類研究会
    • Place of Presentation
      岡山大学 資源植物科学研究所
    • Year and Date
      2016-12-10
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Spatial difference in capsaicinoid biosynthesis gene expression explains higher capsaicinoid contents in chili peppers2016

    • Author(s)
      Y. Tanaka, E. Kirii, F. Nakashima, T. Goto, Y. Yoshida, K. Yasuba
    • Organizer
      11th JKUAT Scientific and Technological Conference
    • Place of Presentation
      Kenya, Jomo Kenyatta University of Agriculture and Technology
    • Year and Date
      2016-11-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Multiple mutated putative aminotransferase alleles contribute to low pungency and capsinoid biosynthesis in Capsicum chinense2016

    • Author(s)
      Y.Tanaka, Y.Muraga, S. Fukuta, T. Goto, Y. Yoshida, K. Yasuba
    • Organizer
      Eucarpia Capsicum and Eggplant Meeting
    • Place of Presentation
      Kecskemet,Hungary
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Genetic analysis of capsaicinoid and its low pungent analogues in chilli pepper(Capsicum chinense)2016

    • Author(s)
      Kirii, Erasmus・後藤丹十郎・吉田裕一・安場健一郎・田中義行
    • Organizer
      平成28年度園芸学会秋季大会
    • Place of Presentation
      名城大学
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
  • [Presentation] トウガラシCapsicum chinenseにおけるpamt遺伝子の構造変異とその辛味性への寄与2016

    • Author(s)
      田中義行・村賀湧次・後藤丹十郎・吉田裕一・安場健一郎
    • Organizer
      平成28年度園芸学会春季大会
    • Place of Presentation
      東京農業大学厚木キャンパス
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 辛味成分高含有トウガラシ系統(C.chinense)におけるカプサイシノイド生合成遺伝子の発現解析2015

    • Author(s)
      田中義行・中島史裕・後藤丹十郎・吉田裕一・安場健一郎
    • Organizer
      平成27年度園芸学会秋季大会
    • Place of Presentation
      徳島大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] A study on Pun1 locus in sweet pepper, cv. ‘Murasaki’ (Capsicum annuum), relative to loss of pungency.2015

    • Author(s)
      キリーエラスムス・田中義行・米田祥二・後藤丹十郎・吉田裕一・安場健一郎
    • Organizer
      平成27年度園芸学会秋季大会
    • Place of Presentation
      徳島大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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