Revealing the mechanisms of nutrient status dependent Plant growth promotion effects by endophytic Colletotrichum species

• Project/Area Number: 15K18645
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Hiruma Kei 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (20714504)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 内生糸状菌 / 植物生長促進 / Colletotrichum / シロイヌナズナ / リン / 根 / 炭疽病菌 / リン欠乏 / Colletotorichum / PHR1 / 植物成長促進 / リン酸 / トリプトファン / リン酸トランスポーター

Outline of Final Research Achievements

Various endophytic fungi associate with plants in nature, but it is unclear whether these provide host fitness benefits. Although most characterized species of the fungal genus Colletotrichum are pathogens causing anthracnose diseases on various plant species, I found that C. tofieldiae (Ct) colonizes roots of brassica plants including Arabidopsis without causing disease symptoms. Ct transfers phosphorus to shoots, promotes plant growth only under phosphorus-limiting conditions. The host’s phosphate starvation response (PSR) system regulates Ct root colonization and is required for plant growth promotion (PGP). PGP also requires a host tryptophan-derived metabolites, components of plant immune responses. These results indicate a functional link between immunity and the PSR system during beneficial interactions with Ct.

Research Products

• [Int'l Joint Research] Max Planck institute/Cologne University(ドイツ)
• [Journal Article] 栄養枯渇条件下での根圏微生物による植物生長促進 (2017)
  • Author(s): 晝間敬、西條雄介
  • Journal Title: 日本植物病理学会会報 （印刷中） Volume: 印刷中
  • Acknowledgement Compliant
• [Journal Article] Root Endophyte Colletotrichum tofieldiae Confers Plant Fitness Benefits that Are Phosphate Status Dependent (2016)
  • Author(s): Hiruma, K., Gerlach, N., Sacristan S., Nakano RT., Hacquard S., Kracher B., Neumann U., Ramirez D., Bucher M., O’Connell RJ and Schulze-Lefert P.
  • Journal Title: Cell Volume: 165 Issue: 2 Pages: 464-474
  • DOI: 10.1016/j.cell.2016.02.028
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Survival trade-offs in plant roots during colonization by closely related beneficial and pathogenic fungi. (2016)
  • Author(s): Hacquard S, Kracher B, Hiruma K, Munch PC, Garrido-Oter R, Thon MR, Weimann A, Damm U, Dallery JF, Hainaut M, Henrissat B, Lespinet O, Sacristan S, Ver Loren van Themaat E, Kemen E, McHardy AC, Schulze-Lefert P, O'Connell RJ.
  • Journal Title: Nature Communications Volume: in press Issue: 1
  • DOI: 10.1038/ncomms11362
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Phosphate Status-dependent control of interactions with pathogenic and endophytic fungi in Arabidopsis thaliana (2017)
  • Author(s): Kei Hiruma, Midori Tanaka, Tae-Hong Lee, Nozomi Kitagawa, Paul Schulze-Lefert, Yusuke Saijo
  • Organizer: Fungal Genetics Conference
  • Place of Presentation: アメリカ・アシロマー
  • Year and Date: 2017-03-13
  • Int'l Joint Research
• [Presentation] リン栄養条件依存的な内生糸状菌との相互作用に関するシロイヌナズナの種内変異 (2017)
  • Author(s): 北川のぞみ、種田有加里、晝間敬、西條雄介
  • Organizer: 植物生理学会
  • Place of Presentation: 鹿児島・鹿児島大学
• [Presentation] Colletotrichum属の内生糸状菌とアブラナ科植物間での栄養条件依存的な共生関係 (2016)
  • Author(s): 晝間敬、西條雄介、Paul Schulze-Lefert
  • Organizer: 植物病理学会感染生理談話会
  • Place of Presentation: 神戸・須磨・シーパル須磨
  • Year and Date: 2016-08-10
  • Invited
• [Presentation] 内生糸状菌 Colletotrichum tofieldiae による植物へのリン酸輸送にはシロイヌナズナの PHR1 及びPHL1 が必要である (2016)
  • Author(s): 晝間敬、 種田有加里、Gerlach Nina, Bucher Marcel, 久保田裕生、Schulze-Lefert Paul、西條雄介.
  • Organizer: 日本植物病理学会全国大会
  • Place of Presentation: 岡山コンベンションセンター（岡山県岡山市）
  • Year and Date: 2016-03-21
• [Presentation] Phosphate dependent plant growth promotion by the root endophyte Colletotrichum tofieldiae (2016)
  • Author(s): Hiruma K, Gerlach N, Sacristan S, Nakano RT, Oida Y, Hacquard S, Kracher B, Bucher M, Saijo Y, O'Connell R, Schulze-Lefert P.
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] 内生糸状菌Colletotrichum tofieldiaeに対する栄養条件依存的な植物免疫の初期応答の解析 (2015)
  • Author(s): 晝間敬、種田有加里、久保田裕生、Schulze-Lefert Paul、西條雄介.
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: あわぎんホール（徳島県徳島市）
  • Year and Date: 2015-09-29
• [Remarks] ライフサイエンス 新着論文レビュー
  • URL: http://first.lifesciencedb.jp/