OsRLCK185 targeted by Xanthomonas effector directly regulates MAPK cascade activated by OsCERK1-mediated recognition of chitin in rice
Project/Area Number |
15K18649
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Plant protection science
|
Research Institution | Kindai University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | イネ白葉枯病菌 / エフェクター / MAPKカスケード / イネ / RLCK / OsRLCK185 / XopY / リン酸化 |
Outline of Final Research Achievements |
Perception of microbe-associated molecular patterns by host cell-surface pattern recognition receptors triggers the intracellular activation of mitogen-activated protein kinase (MAPK) cascades. Plant bacterial pathogens generally deliver different effector proteins into plant cells. These effector proteins modulate the function of crucial host regulatory molecules and allow bacteria to invade plant cells. So far, we identified OsRLCK185 as interacting proteins of Xanthomonas effector, XopY. To understand the molecular mechanism of chitin-induced MAPK activation by OsRLCK185, we searched OsRLCK185 interactor and found MAPK kinase kinase OsMAPKKK18. Chitin-induced MAPK activation in the OsMAPKKK11/18 RNAi cells was clearly reduced compared with WT. Thus, it is likely that OsRLCK185 is the component that directly activates OsMAPKKK18 which triggers the activation of MAPK cascade after chitin perception.
|
Report
(3 results)
Research Products
(11 results)
-
-
[Journal Article] The Arabidopsis CERK1-associated kinase PBL27 connects chitin perception to MAPK activation2016
Author(s)
Yamada K, Yamaguchi K, Shirakawa T, Nakagami H, Mine A, Ishikawa K, Fujiwara M, Narusaka M, Narusaka Y, Ichimura K, Kobayashi Y, Matsui H, Nomura Y, Nomoto M, Tada Y, Fukao Y, Fukamizo T, Tsuda K, Shirasu K, Shibuya N, Kawasaki T
-
Journal Title
The EMBO Journal
Volume: 35(22)
Issue: 22
Pages: 2468-2483
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
-
[Presentation] The CERK1-associated kinase PBL27 regulates chitin-triggered MAPK activation through phosphorylation of MAPKKK5 in Arabidopsis2016
Author(s)
Yamaguchi K, Yamada K, Shirakawa T, Mine A, Kobayashi Y, Fujiwara M, Ichimura K, Narusaka M, Narusaka Y, Tsuda K, Kawasaki T.
Organizer
Cold Spring Harbor Asia Conference, Latest advances in plant development & environmental response
Place of Presentation
兵庫県淡路市 淡路夢舞台国際会議場
Year and Date
2016-11-29
Related Report
Int'l Joint Research
-
[Presentation] PBL27 directly connects between the chitin receptor, CERK1 and MAPK cascade in chitin-triggered immunity.2016
Author(s)
Yamaguchi K, Yamada K, Shirakawa T, Mine A, Narusaka M, Narusaka Y, Ichimura K, Tsuda K, Tamo F, Shibuya N, Kawasaki T.
Organizer
2016 IS-MPMI XVII Congress
Place of Presentation
アメリカ、オレゴン州、ポートランド OREGON CONVENTION CENTER
Year and Date
2016-07-16
Related Report
Int'l Joint Research
-
-
-