Analysis of leaf-like flower development in foxtail millet induced by downy mildew

• Project/Area Number: 15K18650
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: Kobayashi Michie 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 研究員 (10751539)
• Research Collaborator: HIRAKA Yukie (公財)岩手生物工学研究センター, 技術員
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 植物病原菌ゲノム / 病原性エフェクター / 感染生理 / エフェクター / べと病菌 / アワ / 植物病原菌のゲノム解析 / 植物病原菌の全ゲノム解読 / 花成抑制因子

Outline of Final Research Achievements

Sclerospora graminicola is an economically important pathogen of foxtail millet. Colonization of the pathogen in panicles triggers a transformation of florets into leafy structures. Whole genome sequencing of S. graminicola revealed that estimated genome size was approx. 360 Mbp and 16736 genes were predicted from the RNA-seq data. Of a total of 1220 genes encoding putative secreted proteins, including a number of Jacalin-related lectin (JRL) proteins in addition to conserved effector-like proteins. A number of JRLs were induced in early infection phase and seven were detected in apoplastic fluids from infected leaves, suggesting that they play roles as apoplastic effector.
In infected panicles, MADS47 expression was significantly increased despite during reproductive phase. On the other hand, some of positive regulator of floral development decreased protein accumulation. It is suggested that these effects trigger a transformation of flowers into leafy structures.

Research Products

• [Journal Article] Genome analysis of the foxtail millet pathogen Sclerospora graminicola reveals the complex effector repertoire of graminicolous downy mildews (2017)
  • Author(s): Michie Kobayashi, Yukie Hiraka, Akira Abe, Hiroki Yaegashi, Satoshi Natsume, Hideko Kikuchi, Hiroki Takagi, Hiromasa Saitoh, Joe Win, Sophien Kamoun, Ryohei Terauchi
  • Journal Title: BMC Genomics Volume: 18 Issue: 1
  • DOI: 10.1186/s12864-017-4296-z
  • NAID: 120006645322
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] アワしらが病菌新規エフェクター候補の解析 (2018)
  • Author(s): 小林光智衣・平賀幸江・阿部陽・八重樫弘樹・夏目俊・菊池秀子・寺内良平
  • Organizer: 第58回日本植物生理学会年会
• [Presentation] アワしらが病菌ではJacalin様レクチンが独自に進化している (2017)
  • Author(s): 小林光智衣・平賀幸江・阿部陽・八重樫弘樹・夏目俊・菊池秀子・齋藤宏昌・寺内良平
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学 郡元キャンパス
  • Year and Date: 2017-03-16
• [Presentation] アワしらが病菌の全ゲノムシーケンス解析 (2016)
  • Author(s): 小林光智衣
  • Organizer: 農学中手の会
  • Place of Presentation: 雄琴温泉 湯の宿 木もれび
  • Year and Date: 2016-11-10
• [Presentation] Whole genome sequencing of the oomycete pathogen Sclerospora graminicola and prediction of effector candidates (2016)
  • Author(s): Michie Kobayashi, Yukie Hiraka, Akira Abe, Hiroki Yaegashi, Satoshi Natsume, Hideko Kikuchi, Hiroki Takagi, Hiromasa Saitoh, Joe Win, Sophien Kamoun, Ryohei Terauchi
  • Organizer: 2016 IS-MPMI XVII Congress
  • Place of Presentation: the Oregon Convention Center
  • Year and Date: 2016-07-17
  • Int'l Joint Research
• [Presentation] アワしらが病菌の全ゲノムシーケンス解析によるエフェクター候補遺伝子の探索 (2016)
  • Author(s): 小林光智衣・平賀幸江・植村亜衣子・阿部陽・高木宏樹・齋藤宏昌・寺内良平
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-21
• [Presentation] アワしらが病ゲノムの解析 (2015)
  • Author(s): 小林光智衣・平賀幸江・八重樫弘樹・夏目俊・阿部陽・高木宏樹・齋藤宏昌・寺内良平
  • Organizer: 平成27年度植物感染生理談話会
  • Place of Presentation: メルパルク松山
  • Year and Date: 2015-08-24