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Screening of superoxide anion-producing elicitor from biocontrol microorganisms

Research Project

Project/Area Number 15K18653
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant protection science
Research InstitutionNagoya University

Principal Investigator

SATO Ikuo  名古屋大学, 生命農学研究科, 助教 (70743102)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsエリシター / Paenibacillus / スーパーオキシド / キシラナーゼ / 生物防除 / トマト / 生物防除微生物
Outline of Final Research Achievements

We screened for an elicitor from two Paenibacillus strains that have been reported as biocontrol agents against Fusarium crown root rot of tomato. Assays of elicitor activity were conducted with superoxide anion (O2-) producing activity using tomato leaf disc; culture filtrates and cell fractions of the strains had elicitor activity, but these activities were unstable. Apart from this, a xylanase purified from the culture filtrates of Paenibacillus strain had O2- producing activity. In addition, gene expression of pathogenesis related protein in tomato roots were up-regulated by root treatment of the xylanase, suggesting that the enzyme functioned as an elicitor.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] 植物の生体防御機構における一酸化窒素の機能2016

    • Author(s)
      川北一人・佐藤育男
    • Journal Title

      生化学

      Volume: 88-2

    • NAID

      40020843346

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] トマト植物体および栽培土壌からのフザリン酸分解微生物の分離と系統分布2016

    • Author(s)
      澤田祐次・鈴木 萌・千葉壮太郎・竹本大吾・川北一人・佐藤育男
    • Organizer
      日本植物病理学会
    • Place of Presentation
      静岡
    • Year and Date
      2016-09-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] Denitrification of a soil-born phytopathogenic fungus is involved in its pathogenicity2016

    • Author(s)
      長谷部量紀・千葉壮太郎・竹本大吾・ 川北一人・佐藤育男
    • Organizer
      International Symposium on Microbial Ecology
    • Place of Presentation
      Canada
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The dissimilatory nitrite reductase involved in the plant pathogenicity of Fusarium oxysporum f. sp. lycopersici2016

    • Author(s)
      長谷部量紀・千葉壮太郎・竹本大吾・ 川北一人・佐藤育男
    • Organizer
      国際NO学会
    • Place of Presentation
      仙台
    • Year and Date
      2016-05-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] トマト萎凋病菌の異化型亜硝酸還元酵素と病原性2016

    • Author(s)
      長谷部量紀・千葉壮太郎・竹本大吾・川北一人・佐藤育男
    • Organizer
      日本植物病理学会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-03-21
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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