| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
I studied the life cycle in Limax maximus in Ibaraki prefecture, Japan. Slugs with a developed penis were found through a whole year. The development of gonad and albumen gland were seasonally changed. We found that L. maximus start reproduction in fall. To study the effect of photoperiod on reproductive maturation juvenile slugs were reared under short-day or long-day conditions for 240 days and long-day conditions induced gonad maturation. I did not succeed in clarify the relationship between L. maximus to other slugs. L. maximus were collected in in Eastern Japan and Hokkaido to obtain mitochondrial DNA sequence (COI). The data were compared with those from slugs collected in other countries. Several haplotypes were found in Japanese population. Therefore, this result show that several individuals were introduced to Japan.
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