Comprehensive analysis of molecular basis for Mn homeostasis in plants

• Project/Area Number: 15K18660
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: Kochi University
• Principal Investigator: Ueno Daisei 高知大学, 教育研究部総合科学系生命環境医学部門, 准教授 (90581299)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: マンガン / ホメオスタシス / イネ / 耐性 / 集積性 / 吸収 / トランスポーター / 輸送体 / 恒常性 / CDF

Outline of Final Research Achievements

This study aimed to elucidate the molecular basis for Mn homeostasis in rice, which is Mn accumulating and tolerant crop. The plasma membrane-localized MTP9 was localized to the proximal side of endodermal and exodermal cells of roots. This exporter was involved in efficient root Mn uptake in co-operation with NRAMP5, an influx transporter localized at the distal side of the same cell layers. On the other hand, MTP8.2 was tonoplast-localized transporter expressed in both shoots and roots, and involved in the high Mn tolerance. MTP11 was expressed in old leaf blades, which accumulated more Mn than other tissues, at vegetative growth stage. MTP11 was localized to trans-Golgi and mediates Mn tolerance to a lesser extent than tonoplast-localized transporters. At reproductive stage, MTP11 was expressed in reproductive organs and might contribute to high fertility in submerged paddy with high Mn availability.

Research Products

• [Journal Article] A member of cation diffusion facilitator family, MTP11, is required for manganese tolerance and high fertility in rice (2018)
  • Author(s): Tsunemitsu Yuta、Genga Mayuko、Okada Tomoyuki、Yamaji Naoki、Ma Jian Feng、Miyazaki Akira、Kato Shin-ichiro、Iwasaki Kozo、Ueno Daisei
  • Journal Title: Planta Volume: 印刷中 Issue: 1 Pages: 231-241
  • DOI: 10.1007/s00425-018-2890-1
  • Peer Reviewed / Open Access
• [Journal Article] Rice reduces Mn uptake in response to Mn stress (2018)
  • Author(s): Tsunemitsu Yuta、Yamaji Naoki、Ma Jian Feng、Kato Shin-ichiro、Iwasaki Kozo、Ueno Daisei
  • Journal Title: Plant Signaling & Behavior Volume: 13 Issue: 1 Pages: e1422466-e1422466
  • DOI: 10.1080/15592324.2017.1422466
  • Peer Reviewed
• [Journal Article] The Tonoplast-Localized Transporter MTP8.2 Contributes to Manganese Detoxification in the Shoots and Roots of Oryza sativa L. (2017)
  • Author(s): Takemoto Yuma、Tsunemitsu Yuta、Fujii-Kashino Miho、Mitani-Ueno Namiki、Yamaji Naoki、Ma Jian Feng、Kato Shin-ichiro、Iwasaki Kozo、Ueno Daisei
  • Journal Title: Plant and Cell Physiology Volume: 58 Issue: 9 Pages: 1573-1582
  • DOI: 10.1093/pcp/pcx082
  • Peer Reviewed
• [Journal Article] A polarly localized transporter for efficient manganese uptake in rice. (2015)
  • Author(s): Ueno D., Sasaki A., Yamaji N., Miyaji T., Fujii Y., Takemoto Y., Moriyama S., Che J., Moriyama Y., Iwasaki K., Ma J.F.
  • Journal Title: Nature Plants Volume: 1 Issue: 12 Pages: 15170-15170
  • DOI: 10.1038/nplants.2015.170
  • Peer Reviewed / Open Access
• [Presentation] イネの高マンガン集積に関わる分子機構 (2018)
  • Author(s): 上野大勢・常光優太・山地直樹・馬建鋒・岩崎貢三
  • Organizer: 第129回日本森林学会大会
  • Invited
• [Presentation] 植物の重金属輸送に関する研究 (2017)
  • Author(s): 上野大勢
  • Organizer: 日本土壌肥料学会2017年度仙台大会
• [Presentation] トランスゴルジ局在型MTP11はイネのマンガン恒常性に関与する (2017)
  • Author(s): 常光優太・宮崎彰・山地直樹・馬建鋒・加藤伸一郎・岩崎貢三・上野大勢
  • Organizer: 2017年度(第113回)日本土壌肥料学会関西支部講演会
• [Presentation] The role of cation diffusion facilitators in manganese homeostasis in rice (2016)
  • Author(s): Daisei Ueno
  • Organizer: Workshop on Soil-Microbe-Plant Interaction_2016
  • Place of Presentation: 岡山大学資源植物科学研究所
  • Year and Date: 2016-12-03
  • Int'l Joint Research / Invited
• [Presentation] 液胞膜型輸送体MTP8.1とMTP8.2はイネの根におけるマンガン耐性に寄与する (2016)
  • Author(s): 常光優太、竹本侑馬、山地直樹、馬建鋒、加藤伸一郎、岩崎貢三、上野大勢
  • Organizer: 植物化学調節学会第51回大会
  • Place of Presentation: 高知大学
  • Year and Date: 2016-10-28
• [Presentation] 植物のマンガン恒常性を司る輸送システム (2016)
  • Author(s): 上野大勢
  • Organizer: 第11回トランスポーター研究会年会
  • Place of Presentation: 京都大学 宇治キャンパス きはだホール
  • Year and Date: 2016-07-02
  • Invited
• [Presentation] 作物の鉄欠乏改善への人工シデロフォア―鉄錯体の利用 (2015)
  • Author(s): 寺内亨, 渡邊武士, 鶴薗克敏, 岩崎貢三, 上野大勢, 松本健司
  • Organizer: 2015年度日本土壌肥料学会関西支部講演会
  • Place of Presentation: メルパルク松山
  • Year and Date: 2015-12-11
• [Presentation] MTP8とMTP8.1はイネの根におけるマンガン耐性に寄与する (2015)
  • Author(s): 常光優太, 竹本侑馬, 山地直樹, 馬建鋒, Mayila Yusuyin, 加藤伸一郎, 岩崎貢三, 上野大勢
  • Organizer: 日本土壌肥料学会2015年度京都大会
  • Place of Presentation: 京都大学
  • Year and Date: 2015-09-09
• [Presentation] 植物の重金属吸収システム (2015)
  • Author(s): 上野大勢
  • Organizer: 平成27年度高知化学会
  • Place of Presentation: 高知大学
  • Year and Date: 2015-08-30
  • Invited
• [Presentation] Vacuolar sequestration of Mn in leaf epidermis by OsMTP8 is required for Mn tolerance in rice (2015)
  • Author(s): D. Ueno, Y. Takemoto, S. Kotake, N. Yamaji, J.F. Ma, S. Kato, K. Iwasaki
  • Organizer: 13th International Conference on the Biogeochemistry of Trace Elements
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2015-07-12
  • Int'l Joint Research
• [Presentation] Molecular mechanisms of cadmium accumulation in cereal crops (2015)
  • Author(s): J.F. Ma, N. Yamaji, A. Sasaki, D. Ueno, D. Wu
  • Organizer: 13th International Conference on the Biogeochemistry of Trace Elements
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2015-07-12
  • Int'l Joint Research
• [Remarks] 農林海洋科学部の上野大勢准教授が第35回日本土壌肥料学会奨励賞を受賞しました
  • URL: http://www.kochi-u.ac.jp/information/2017090800012/