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Comprehensive analysis of molecular basis for Mn homeostasis in plants

Research Project

Project/Area Number 15K18660
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionKochi University

Principal Investigator

Ueno Daisei  高知大学, 教育研究部総合科学系生命環境医学部門, 准教授 (90581299)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsマンガン / ホメオスタシス / イネ / 耐性 / 集積性 / 吸収 / トランスポーター / 輸送体 / 恒常性 / CDF
Outline of Final Research Achievements

This study aimed to elucidate the molecular basis for Mn homeostasis in rice, which is Mn accumulating and tolerant crop. The plasma membrane-localized MTP9 was localized to the proximal side of endodermal and exodermal cells of roots. This exporter was involved in efficient root Mn uptake in co-operation with NRAMP5, an influx transporter localized at the distal side of the same cell layers. On the other hand, MTP8.2 was tonoplast-localized transporter expressed in both shoots and roots, and involved in the high Mn tolerance. MTP11 was expressed in old leaf blades, which accumulated more Mn than other tissues, at vegetative growth stage. MTP11 was localized to trans-Golgi and mediates Mn tolerance to a lesser extent than tonoplast-localized transporters. At reproductive stage, MTP11 was expressed in reproductive organs and might contribute to high fertility in submerged paddy with high Mn availability.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016 2015 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (11 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results) Remarks (1 results)

  • [Journal Article] A member of cation diffusion facilitator family, MTP11, is required for manganese tolerance and high fertility in rice2018

    • Author(s)
      Tsunemitsu Yuta、Genga Mayuko、Okada Tomoyuki、Yamaji Naoki、Ma Jian Feng、Miyazaki Akira、Kato Shin-ichiro、Iwasaki Kozo、Ueno Daisei
    • Journal Title

      Planta

      Volume: 印刷中 Issue: 1 Pages: 231-241

    • DOI

      10.1007/s00425-018-2890-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rice reduces Mn uptake in response to Mn stress2018

    • Author(s)
      Tsunemitsu Yuta、Yamaji Naoki、Ma Jian Feng、Kato Shin-ichiro、Iwasaki Kozo、Ueno Daisei
    • Journal Title

      Plant Signaling & Behavior

      Volume: 13 Issue: 1 Pages: e1422466-e1422466

    • DOI

      10.1080/15592324.2017.1422466

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Tonoplast-Localized Transporter MTP8.2 Contributes to Manganese Detoxification in the Shoots and Roots of Oryza sativa L.2017

    • Author(s)
      Takemoto Yuma、Tsunemitsu Yuta、Fujii-Kashino Miho、Mitani-Ueno Namiki、Yamaji Naoki、Ma Jian Feng、Kato Shin-ichiro、Iwasaki Kozo、Ueno Daisei
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 9 Pages: 1573-1582

    • DOI

      10.1093/pcp/pcx082

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A polarly localized transporter for efficient manganese uptake in rice.2015

    • Author(s)
      Ueno D., Sasaki A., Yamaji N., Miyaji T., Fujii Y., Takemoto Y., Moriyama S., Che J., Moriyama Y., Iwasaki K., Ma J.F.
    • Journal Title

      Nature Plants

      Volume: 1 Issue: 12 Pages: 15170-15170

    • DOI

      10.1038/nplants.2015.170

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] イネの高マンガン集積に関わる分子機構2018

    • Author(s)
      上野大勢・常光優太・山地直樹・馬建鋒・岩崎貢三
    • Organizer
      第129回日本森林学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 植物の重金属輸送に関する研究2017

    • Author(s)
      上野大勢
    • Organizer
      日本土壌肥料学会2017年度仙台大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トランスゴルジ局在型MTP11はイネのマンガン恒常性に関与する2017

    • Author(s)
      常光優太・宮崎彰・山地直樹・馬建鋒・加藤伸一郎・岩崎貢三・上野大勢
    • Organizer
      2017年度(第113回)日本土壌肥料学会関西支部講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The role of cation diffusion facilitators in manganese homeostasis in rice2016

    • Author(s)
      Daisei Ueno
    • Organizer
      Workshop on Soil-Microbe-Plant Interaction_2016
    • Place of Presentation
      岡山大学資源植物科学研究所
    • Year and Date
      2016-12-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 液胞膜型輸送体MTP8.1とMTP8.2はイネの根におけるマンガン耐性に寄与する2016

    • Author(s)
      常光優太、竹本侑馬、山地直樹、馬建鋒、加藤伸一郎、岩崎貢三、上野大勢
    • Organizer
      植物化学調節学会第51回大会
    • Place of Presentation
      高知大学
    • Year and Date
      2016-10-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 植物のマンガン恒常性を司る輸送システム2016

    • Author(s)
      上野大勢
    • Organizer
      第11回トランスポーター研究会年会
    • Place of Presentation
      京都大学 宇治キャンパス きはだホール
    • Year and Date
      2016-07-02
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 作物の鉄欠乏改善への人工シデロフォア―鉄錯体の利用2015

    • Author(s)
      寺内亨, 渡邊武士, 鶴薗克敏, 岩崎貢三, 上野大勢, 松本健司
    • Organizer
      2015年度日本土壌肥料学会関西支部講演会
    • Place of Presentation
      メルパルク松山
    • Year and Date
      2015-12-11
    • Related Report
      2015 Research-status Report
  • [Presentation] MTP8とMTP8.1はイネの根におけるマンガン耐性に寄与する2015

    • Author(s)
      常光優太, 竹本侑馬, 山地直樹, 馬建鋒, Mayila Yusuyin, 加藤伸一郎, 岩崎貢三, 上野大勢
    • Organizer
      日本土壌肥料学会2015年度京都大会
    • Place of Presentation
      京都大学
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 植物の重金属吸収システム2015

    • Author(s)
      上野大勢
    • Organizer
      平成27年度高知化学会
    • Place of Presentation
      高知大学
    • Year and Date
      2015-08-30
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Vacuolar sequestration of Mn in leaf epidermis by OsMTP8 is required for Mn tolerance in rice2015

    • Author(s)
      D. Ueno, Y. Takemoto, S. Kotake, N. Yamaji, J.F. Ma, S. Kato, K. Iwasaki
    • Organizer
      13th International Conference on the Biogeochemistry of Trace Elements
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-07-12
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular mechanisms of cadmium accumulation in cereal crops2015

    • Author(s)
      J.F. Ma, N. Yamaji, A. Sasaki, D. Ueno, D. Wu
    • Organizer
      13th International Conference on the Biogeochemistry of Trace Elements
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-07-12
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 農林海洋科学部の上野大勢准教授が第35回日本土壌肥料学会奨励賞を受賞しました

    • URL

      http://www.kochi-u.ac.jp/information/2017090800012/

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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