The epigenetics-driven adaptive evolution concept and its application to microbe engineering

• Project/Area Number: 15K18665
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: University of Tsukuba (2016-2017); The University of Tokyo (2015)
• Principal Investigator: Yano Hirokazu 筑波大学, 生命環境系, 研究員 (20646773)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ゲノム / ピロリ菌 / メチローム / トランスクリプトーム / 制限修飾系 / エピゲノム

Outline of Final Research Achievements

We revealed the functionality of diverse methyltransferase genes in the pathogen (H. pylori) genome. We established experimental and analytical protocols for bacterial methylome and transcriptome studies. We discovered an epigenetic network consisting of multiple regulatory networks governed by site-specific DNA methyltransferases. We discovered a novel restriction-modification system potentially useful for epigenome engineering.

Research Products

• [Journal Article] Restriction glycosylases: involvement of endonuclease activities in the restriction process. (2017)
  • Author(s): Ming-Zhang Xie, Tomoyuki Matsuzaka, Hirokazu Yano, Yoshikazu Furuta, Toshiaki Nakano, Ken Ishikawa, Masaki Fukuyo, Noriko Takahashi, Yutaka Suzuki, Sumio Sugano, Hiroshi Ide and Ichizo Kobayashi
  • Journal Title: Nucleic Acids Res Volume: 45 Pages: 1392-1403
  • DOI: 10.1093/nar/gkw1250
  • Peer Reviewed / Open Access
• [Presentation] Population structure and local adaptation of MAC lung disease agent Mycobacterium avium subsp. hominissuis (2018)
  • Author(s): Hirokazu Yano, Tomotada Iwamoto, Yukiko Nishiuchi, Chie Nakajima, Daria A. Starkova, Igor Mokrousov, Olga Narvskaya, Shiomi Yoshida, Kentaro Arikawa, Noriko Nakanishi, Ken Osaki, Ichiro Nakagawa, Manabu Ato, Yasuhiko Suzuki, and Fumito Maruyama
  • Organizer: 日本進化学会 京都大学吉田キャンパス 2017年８月24-26日
• [Presentation] Population structure and local adaptation of MAC lung disease agent Mycobacterium avium subsp. hominissuis (2018)
  • Author(s): Hirokazu Yano, Tomotada Iwamoto, Yukiko Nishiuchi, Chie Nakajima, Daria A. Starkova, Igor Mokrousov, Olga Narvskaya, Shiomi Yoshida, Kentaro Arikawa, Noriko Nakanishi, Ken Osaki, Ichiro Nakagawa, Manabu Ato, Yasuhiko Suzuki, and Fumito Maruyama
  • Organizer: The 52nd US-Japan Mycobacteria Panel Meeting 2018 in Niigata, Japan, March 15-16, 2018
  • Int'l Joint Research
• [Presentation] 種内相互組み換えによって促進される鳥型結核菌の地域適応 (2017)
  • Author(s): 矢野大和、丸山史人、西内由紀子、中川一路、中島千絵、鈴木定彦、岩本朋忠
  • Organizer: 日本ゲノム微生物学会2017年度大会
  • Place of Presentation: 慶応大学湘南藤沢キャンパス（神奈川県藤沢市）
  • Year and Date: 2017-03-02
• [Presentation] III 型制限修飾系によるピロリ菌の適応形質とゲノミックアイランドの制御 (2016)
  • Author(s): 矢野大和、林原恵美子、古田芳一、柴山恵吾、小林一三
  • Organizer: 第22回ヘリコバクター学会学術集会
  • Place of Presentation: 別府国際コンベンションセンターB-CON PLAZA (大分県別府市)
  • Year and Date: 2016-06-24
• [Presentation] ピロリ菌の適応形質を支配するDNAメチル化系ネットワークの発見 (2016)
  • Author(s): 矢野大和、M Zobaidul Alam, 林原絵美子、古田芳一、鈴木穣、菅野純夫、柴山恵吾、小林一三
  • Organizer: 日本農芸化学会 2016年度大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-30
• [Presentation] Epigenetic network of gene expression involving DNA methyltransferases (2016)
  • Author(s): Hirokazu Yano, Md Zobaidul Alam, Emirko Rimbara, Yoshikazu Furuta, Yutaka Suzuki, Sumio Sugano, Keigo Shibayama, Ichizo Kobayahsi
  • Organizer: 第１０会ゲノム微生物学会
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 2016-03-03
• [Presentation] メチローム変換に基づく進化 (2015)
  • Author(s): 矢野大和、古田芳一、Zobaidu M. Alam、林原絵美子、柴田朋子、西山智明、重信秀治、長谷部光泰、鈴木穰、菅野純夫、柴山恵吾、小林一三
  • Organizer: 日本遺伝学会第８６回大会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2015-09-24
  • Invited
• [Presentation] ピロリ菌の適応形質を支配するDNAメチル化系ネットワークの発見 (2015)
  • Author(s): 矢野大和、Zobaidul M. Alam、林原絵美子、古田芳一、鈴木穣、菅野純夫、柴山恵吾、小林一三
  • Organizer: 日本進化学会第１７回大会
  • Place of Presentation: 中央大学後楽園キャンパス
  • Year and Date: 2015-08-20
• [Presentation] Network of DNA methyltransferase behind the adaptive phenotype in Helicobacteri pylori (2015)
  • Author(s): Hirokazu Yano, Zobaidul M. Alam, Emiko Rimbara, Yoshikazu Furuta, Yutaka Suzuki, Sumio Sugano, Keigo Shibayama, Ichizo Kobayashi
  • Organizer: Gordon Research Conference, Microbial Population Biology
  • Place of Presentation: Andover, NH, USA
  • Year and Date: 2015-07-19
  • Int'l Joint Research
• [Remarks] Hirokazu Yano
  • URL: https://sites.google.com/view/hirokazuyano/home
• [Remarks] Hirokazu Yano
  • URL: https://sites.google.com/site/hymyresearch/