Development of a culture-independent method for the identification of microbial enzyme-encoding genes using water-in-oil microdroplets and its application
Project/Area Number |
15K18668
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
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Research Institution | The University of Tokyo |
Principal Investigator |
Iizuka Ryo 東京大学, 大学院薬学系研究科(薬学部), 助教 (90541954)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 液滴 / 微生物 / 酵素 / マイクロ流体デバイス |
Outline of Final Research Achievements |
Environmental microbes are a great source of industrially valuable enzymes with potent and unique catalytic activities. Unfortunately, the majority of microbes remain unculturable and thus are not accessible by culture-based methods. Then, I envisioned a culture-independent method for the identification of microbial enzyme-encoding genes using water-in-oil microdroplets. In this study, I established the method and demonstrated its practicality and usefulness. This method will facilitate the identification of genes encoding industrially valuable enzymes.
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Report
(3 results)
Research Products
(26 results)
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[Presentation] A single integrated gene nano-chip functioning in an artificial cell2016
Author(s)
Takeya Masubuchi, Masayuki Endo, Ryo Iizuka, Ayaka Iguchi, Yoon Doung Hyun, Tetsushi Sekiguchi, Hao Qi, Ryosuke Iinuma, Yuya Miyazono, Shuichi Shoji, Takashi Funatsu, Hiroshi Sugiyama, Yoshie Harada, Takuya Ueda, Hisashi Tadakuma
Organizer
第54回日本生物物理学会年会
Place of Presentation
つくば国際会議場(茨城県つくば市)
Year and Date
2016-11-25 – 2016-11-27
Related Report
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[Presentation] IN VITRO SELECTION OF NOVEL PEPTIDE AGONISTS FOR HUMAN SOMATOSTATIN RECEPTOR SUBTYPE-2 USING A WATER-IN-OIL MICRODROPLET PLATFORM2016
Author(s)
T. Sakurai, R. Iizuka, Y. Nakamura, J. Ishii, A. Kondo, A. Iguchi, D. H. Yoon, T. Sekiguchi, S. Shoji, T. Funatsu
Organizer
The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Place of Presentation
Convention Center Dublin (Dublin, Ireland)
Year and Date
2016-10-09 – 2016-10-13
Related Report
Int'l Joint Research
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[Presentation] Directed evolution system to generate peptide agonists for G protein-coupled receptors using water-in-oil droplets2016
Author(s)
Ryo Iizuka, Takashi Sakurai, Yasuyuki Nakamura, Jun Ishii, Akihiko Kondo, Rui Sekine, Dong Hyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji, Takashi Funatsu
Organizer
The 8th Takeda Science Foundation Symposium on PharmaSciences Biomolecule-Based Medicinal Science: Featuring Mid-Size Drugs, Center for Learning and Innovation
Place of Presentation
武田薬品研修所(大阪府吹田市)
Year and Date
2016-01-21 – 2016-01-22
Related Report
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[Presentation] CULTURE-INDEPENDENT METHOD FOR SCREENING AND IDENTIFYING MICROBIAL ENZYME-ENCODING GENES USING MICRODROPLET-BASED SINGLE CELL GENOMICS2015
Author(s)
K. Nakamura, R. Iizuka, T. Yoshida, Y. Hatada, Y. Takaki, S. Nishi, A. Iguchi, D. H. Yoon, T. Sekiguchi, S. Shoji, T. Funatsu
Organizer
The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
Place of Presentation
Hwabaek International Convention Center (Gyeongju, Korea)
Year and Date
2015-10-25 – 2015-10-29
Related Report
Int'l Joint Research
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[Presentation] Culture-independent method for identifying microbial enzyme-encoding genes based on activity-driven single cell genomics2015
Author(s)
Kazuki Nakamura, Ryo Iizuka, Takao Yoshida, Yuji Hatada, Yoshihiro Takaki, Shinro Nishi, Ayaka Iguchi, Dong Hyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji, Takashi Funatsu
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学 角間キャンパス 自然科学本館(石川県金沢市)
Year and Date
2015-09-13 – 2015-09-15
Related Report
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