| Project/Area Number |
15K18672
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka University |
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Principal Investigator |
Sasano Yu 大阪大学, 工学研究科, 助教 (90640194)
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| Co-Investigator(Renkei-kenkyūsha) |
HARASHIMA Satoshi 崇城大学, 生物生命学部, 教授 (70116086)
SUGIYAMA Minetaka 大阪大学, 大学院工学研究科, 准教授 (80379130)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | CRISPR-PCS / 出芽酵母 / ゲノム工学 / 染色体工学 / CRISPR/Cas9 / 菌株育種 / CRISPR-Cas / Chromosome engineering / Yeast |
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| Outline of Final Research Achievements |
Most useful traits required for industrial yeast strains are regulated by many genes. Thus, development of genome engineering technology for large scale genome manipulation in yeast is desired. In this study, a novel chromosome engineering technology called CRISPR-PCS that enables simultaneous splitting of yeast chromosomes at the targeted sites at a time was developed. With this technology, highly efficient chromosome splitting became possible and we succeeded in quadruple chromosome splitting. In addition, other chromosomal manipulation such as deletion, duplication, replacement, translocation, fusion, and circularization also became possible by applying CRISPR-PCS. It is expected that CRISPR-PCS technology can be applied to innovative microbial breeding.
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