Exhaustive screening of D-amino acids/rare sugars-metabolizing enzyme genes in subseafloor microbial communities
| Project/Area Number |
15K18674
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Kochi University |
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Principal Investigator |
Wakamatsu Taisuke 高知大学, 教育研究部総合科学系生命環境医学部門, 講師 (60597938)
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| Research Collaborator |
MORONO Yuki
INAGAKI Fumio
ASHIUCHI Makoto
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 機能性遺伝子 / 海底下微生物 / D-アミノ酸 / 希少糖 / 基質誘導 / 遺伝子応答 / メタゲノム / 深海底微生物 |
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| Outline of Final Research Achievements |
I determined the percentage of clones in shotgun metagenomics libraries prepared with subseafloor sediment samples at Nankai Trough and South Pacific Gyre with green fluorescence as the response to various D-amino acids and rare sugars using the SIGEX method. Clones with positive induction response to D-Trp, D-Arg, or D-Asp from the South Pacific Gyre library were sorted by a cell sorter. I determined DNA sequences for these three clones using the Sanger method. Furthermore, I brushed up the method for construction of libraries, and hence, both the length of inserted DNA fragment and the number of clones were significantly improved.
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Report
(3 results)
Research Products
(9 results)
