| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Bacillus subtilis spore is surrounded by a polysaccharide layer. This study was aimed for understanding of the morphological process of formation of the polysaccharide layer. Here, we uncovered the molecular mechanism of activation of spsM, which was identified as an essential gene for the generation of the polysaccharide layer. In search for the other members that are required for the polysaccharide layer production, we identified spsABCDEFGIJKL, cgeAB, cgeCDE, cotVWXYZ, and gerE which encodes the transcriptional factor for their expression. Based on the amino-acids sequences, Sps and Cge proteins are considered to synthesize and elongate the polysaccharides. Furthermore, we revealed that spore coat proteins, CotW, CotX, and CotZ, play the important role in the attachment of the polysaccharides on the spore surface.
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