Study of the formation process of the Bacillus subtilis spore polysaccharide layer for potential applications.
Project/Area Number |
15K18675
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
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Research Institution | Hosei University |
Principal Investigator |
ABE Kimihiro 法政大学, マイクロ・ナノテクノロジー研究センター, 研究員 (10748940)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Keywords | 枯草菌 / 胞子 / 多糖類合成 / 遺伝子再編成 / 部位特異的DNA組換え / ファージ / 多糖類 |
Outline of Final Research Achievements |
Bacillus subtilis spore is surrounded by a polysaccharide layer. This study was aimed for understanding of the morphological process of formation of the polysaccharide layer. Here, we uncovered the molecular mechanism of activation of spsM, which was identified as an essential gene for the generation of the polysaccharide layer. In search for the other members that are required for the polysaccharide layer production, we identified spsABCDEFGIJKL, cgeAB, cgeCDE, cotVWXYZ, and gerE which encodes the transcriptional factor for their expression. Based on the amino-acids sequences, Sps and Cge proteins are considered to synthesize and elongate the polysaccharides. Furthermore, we revealed that spore coat proteins, CotW, CotX, and CotZ, play the important role in the attachment of the polysaccharides on the spore surface.
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Report
(4 results)
Research Products
(43 results)
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[Presentation] 枯草菌胞子最外層の役割2015
Author(s)
岩本敬人、稲井貴志、井之口紫苑、安部公博、佐藤勉
Organizer
グラム陽性菌ゲノム機能会議
Place of Presentation
里湯昔話 雄山荘 (滋賀県・大津市)
Year and Date
2015-08-27
Related Report
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[Presentation] Rearrangement of spsM, a spore polysaccharide synthesis gene in Bacillus subtilis, is mediated by the SPβ site-specific recombination factors, SprA and SprB2015
Author(s)
Kimihiro Abe, Takuo Takamatsu, Yuta Kawano, Keito Iwamoto, Patrick Eichenberger, and Tsutomu Sato
Organizer
8th International Conference on Gram-positive Microorganisms, 18th International Conference on Bacilli
Place of Presentation
Tuscany, Italy
Year and Date
2015-06-21
Related Report
Int'l Joint Research