| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The hetero-oligomeric complex of the FlhD and FlhC (FlhD4C2) plays a pivotal role in the transcriptional regulation of flagellar regulon. The flhD operon has been referred to as the “master operon” because it is required for the expression of level II and level III flagellar and chemotaxis genes. In recent years, it has been shown that FlhD4C2 regulates a number of non-flagellar promoters in E. coli. However, the detail mechanism remains unclear. we tried to identify the regulatory function of FlhD4C2. After SELEX screening, FlhD4C2 was found to bind to not only the promoters of flagellar regulon but also that of genes involved in central carbon metabolism. These results demonstrate the mechanism how bacteria cooperate flagellar formation with central metabolism responding to the environmental changes.
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