| Project/Area Number |
15K18677
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Waseda University |
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Principal Investigator |
HARA Ryotaro 早稲田大学, 理工学術院総合研究所(理工学研究所), 次席研究員(研究院講師) (70553535)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | プロリン / ヒドロキシプロリン / エクトイン / 水酸化酵素 / 微生物変換 / trans-3-ヒドロキシ-L-プロリン / ヒドロキシエクトイン / アミノ酸水酸化酵素 / エクトイン水酸化酵素 / 2-オキソグルタル酸依存型ジオキシゲナーゼ |
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| Outline of Final Research Achievements |
The objective of this study was to develop an enzymatic production process for trans-3-hydroxyproline. Sequence-based screening for a novel proline hydroxylating enzyme approach allowed the identification of a proline trans-3-selective hydroxylation activity in ectoine hydroxylase from Streptomyces cattleya. Using the Escherichia coli expressing the ectoine hydroxylase gene as a biocatalyst, an efficient trans-3-hydroxyproline production was achieved. Further, a novel trans-3-hydroxyproline synthetic route was developed via arginine hydroxylation, hydrolysis, and cyclodeamination in this order.
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