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Modulation of organelle localized ion transporter by lipid molecule.

Research Project

Project/Area Number 15K18678
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionTohoku University

Principal Investigator

Hamamoto Shin  東北大学, 工学研究科, 助教 (10533812)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsイオンチャネル / 出芽酵母 / 液胞 / カルシウム / 酵母 / Ca2+ / トランスポーター / チャネル
Outline of Final Research Achievements

TRPY1 is a major cation channel localized in yeast vacuole membrane. We have applied the patch-clamp technique using enlarged yeast cells to elucidate the regulatory mechanisms of the TRPY1 activity. We have identified the Cys crucial for the functional property of the TRPY1 channel in the presence of reducing agent. Next we examined the effect of phosphatidyl inositol phosphate on TRPY1 function since there are several reports on the involvement of PI(3,5)P2 in hyperosmotic stress induced Ca2+ release from organelle. We assessed whether other phosphatidylinositol phosphates (PIPs) affect to the TRPY1 activity, which is monitored as aequorin-mediated luminescence in yeast mutants defective in phosphorylation and dephosphorylation of PIPs. One of mutant of PIP phosphatase showed decreased activity in hyperosmotic stress induced Ca2+ release. Furthermore, the patch-clamp analysis revealed that addition of PI(3)P to the cytoplasmic side reduced the whole-vacuole currents of TRPY1.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (26 results)

All 2018 2017 2016 2015

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Identification and characterization of compounds that affect stomatal movements.2018

    • Author(s)
      Toh S, Inoue S, Toda Y, Yuki T, Suzuki K, Hamamoto S, Fukatsu K, Aoki S, Uchida M, Asai E, Uozumi N, Sato A, Kinoshita T.
    • Journal Title

      Plant & Cell Physiology

      Volume: in press Issue: 8 Pages: 1568-1580

    • DOI

      10.1093/pcp/pcy061

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] N-myristoylation and S-acylation are common modifications of Ca2+-regulated Arabidopsis kinases and are required for activation of the SLAC1 anion channel2018

    • Author(s)
      Saito Shunya、Hamamoto Shin、Moriya Koko、Matsuura Aiko、Sato Yoko、Muto Jun、Noguchi Hiroto、Yamauchi Seiji、Tozawa Yuzuru、Ueda Minoru、Hashimoto Kenji、Koester Philipp、Dong Qiuyan、Held Katrin、Kudla Joerg、Utsumi Toshihiko、Uozumi Nobuyuki
    • Journal Title

      New Phytologist

      Volume: 218 Issue: 4 Pages: 1504-1521

    • DOI

      10.1111/nph.15053

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In vitro and in vivo characterization of modulation of the vacuolar cation channel TRPY1 from Saccharomyces cerevisiae2018

    • Author(s)
      Shin Hamamoto, Yasuo Mori, Isamu Yabe, Nobuyuki Uozumi
    • Journal Title

      The Febs Journal

      Volume: 285 Issue: 6 Pages: 1146-1161

    • DOI

      10.1111/febs.14399

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dimerization of GTR1 regulates their plasma membrane localization2017

    • Author(s)
      Ishimaru Yasuhiro、Washiyama Kento、Oikawa Takaya、Hamamoto Shin、Uozumi Nobuyuki、Ueda Minoru
    • Journal Title

      Plant Signaling & Behavior

      Volume: 12 Issue: 6 Pages: 1334749-1334749

    • DOI

      10.1080/15592324.2017.1334749

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kup-mediated Cs+ uptake and Kdp-driven K+ uptake coordinate to promote cell growth during excess Cs+ conditions in Escherichia coli2017

    • Author(s)
      Ellen Tanudjaja, Naomi Hoshi, Yi-Hsin Su, Shin Hamamoto & Nobuyuki Uozumi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-9

    • DOI

      10.1038/s41598-017-02164-7

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] GTR1 is a jasmonic acid and jasmonoyl-l-isoleucine transporter in Arabidopsis thaliana.2017

    • Author(s)
      Ishimaru, Y., Oikawa, T., Suzuki, T., Takeishi, S., Matsuura, H., Takahashi, K., Hamamoto, S., Uozumi, N., Shimizu, T., Seo, M., Ohta, H., and Ueda, M.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 81 Issue: 2 Pages: 249-255

    • DOI

      10.1080/09168451.2016.1246174

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] The jasmonate-responsive GTR1 transporter is required for gibberellin-mediated stamen development in Arabidopsis2015

    • Author(s)
      H. Saito, T. Oikawa, S. Hamamoto, Y. Ishimaru, T. Utsumi, J. Chen, M. Kanamori, Y. Sasaki-Sekimoto, M. Shimojima, S. Masuda, Y. Kamiya, M. Seo, N. Uozumi, M. Ueda, H. Ohta, A
    • Journal Title

      Nature Communications

      Volume: 6 Issue: 1 Pages: 6095-6095

    • DOI

      10.1038/ncomms7095

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] HKT transporters mediate salt stress resistance in plants: from structure and function to the field.2015

    • Author(s)
      Hamamoto, S., Horie, T., Hauser, F., Deinlein, U., Schroeder J.I. and Uozumi, N.
    • Journal Title

      Current Opinion in Biotechnology

      Volume: 32 Pages: 113-120

    • DOI

      10.1016/j.copbio.2014.11.025

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 浸透圧ストレスで誘導される大腸菌のフロック形成機構の解析2017

    • Author(s)
      菅原慶太,早坂俊亮,浜本 晋,井原邦夫,斎藤浩美,小林 弘,魚住 信之
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Research-status Report
  • [Presentation] 高浸透圧条件下における大腸菌機械受容チャネルYbdGのC末端領域の機能解析2017

    • Author(s)
      雨宮駿, 豊田隼斗, 浜本晋, 鎌形清人, 古田忠臣, 井原邦夫, 斎藤浩美, 小林弘, 魚住信之
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Research-status Report
  • [Presentation] 出芽酵母のストレス応答に関わる新規4つの陽イオンチャネルの機能解析2017

    • Author(s)
      上原千央、星直美、浜本晋、魚住信之
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Research-status Report
  • [Presentation] Inhibition of Arabidopsis K+ channel by flavonoids2017

    • Author(s)
      遠藤晃輔, 浜本晋, 魚住信之
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] シロイヌナズナのathkt1欠損株におけるNa, K循環と蓄積の解析2017

    • Author(s)
      竹林昂亮,菊永英寿,加藤恵,横山隆亮,西谷和彦,渡部浩司,須田利美,黒森崇,石川敦司,堀江智明,鈴井伸郎,尹永根,河地有木,藤巻秀,樋口恭子,森泉,浜本晋,魚住信之
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Characterization of novel calcium channels in Saccharomyces cerevisiae2017

    • Author(s)
      Asuka Shibata, Shin Hamamoto and Nobuyuki Uozumi
    • Organizer
      Tohoku University's Chemistry Summer School
    • Related Report
      2017 Annual Research Report
  • [Presentation] 芽酵母の新規Ca2+輸送体の機能解析2017

    • Author(s)
      上原千央,浜本晋,星直美,魚住信之
    • Organizer
      第12回トランスポーター研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酵母における新規Ca2+輸送タンパク質の局在性と遺伝子発現の解析2017

    • Author(s)
      柴田あすか, 上原千央, 星直美, 浜本晋, 魚住信之
    • Organizer
      第12回トランスポーター研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酵母液胞TRPの機能と役割とオルガネラ輸送体の大腸菌を用いた解析2017

    • Author(s)
      魚住信之,浜本晋
    • Organizer
      第13回TRPチャネル研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酵母液胞膜局在性TRPチャネルの機能解析と 酵母液胞膜を発現系としたイオンチャネルの解析2017

    • Author(s)
      浜本 晋
    • Organizer
      イオンチャネル研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 大腸菌K取込みトランスポーターKupの機能解析2016

    • Author(s)
      Ellen, 星直美,蘇怡欣,七谷圭,浜本晋,魚住信之
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議城(富山市)
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
  • [Presentation] ホスファチジルイノシトールリン酸による酵母液胞膜Ca<SUP>2+</SUP>チャネルTRPY1の活性調節機構の解析2016

    • Author(s)
      浜本晋, 矢部勇, 森泰生, 魚住信之
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Lipidation of Arabidopsis CPK6 promotes the plasma membrane targeting and the stomata closure mediated by activation of SLAC1 in Arabidopsis guard cells2016

    • Author(s)
      Saito, S., Hamamoto, S., Sato, Y., Uozumi, N., Hashimoto, K., Kudla, J., Utsumi, T., Moriya, K., Tozawa, Y., and Yamauchi, S.
    • Organizer
      International workshop on Plant Membrane Biology
    • Place of Presentation
      Annapolis Maryland, USA
    • Year and Date
      2016-06-05
    • Related Report
      2016 Research-status Report
  • [Presentation] C-terminal cytosolic region of YVC1 is involved in regulation of calcium channel activity in yeast vacuolar membrane2016

    • Author(s)
      Hamamoto, S., Yabe, I. and Uozumi, N.
    • Organizer
      International workshop on Plant Membrane Biology
    • Place of Presentation
      Annapolis Maryland, USA
    • Year and Date
      2016-06-05
    • Related Report
      2016 Research-status Report
  • [Presentation] Wound-inducible GTR1 transporter is involved in JA-Ile translocation during wound response in Arabidopsis2016

    • Author(s)
      Oikawa,T., Ishimaru, Y., Takeishi, S., Matsuura, H., Takahashi, K., Hamamoto, S., Uozumi, N., Shimizu, T., Seo, M., Ohta, H., and Ueda., M.
    • Organizer
      International workshop on Plant Membrane Biology
    • Place of Presentation
      Annapolis Maryland, USA
    • Year and Date
      2016-06-05
    • Related Report
      2016 Research-status Report
  • [Presentation] 酵母の細胞壁合成に関わる新規膜タンパク質の機能解析2016

    • Author(s)
      上原千央,星直美,浜本晋,魚住信之
    • Organizer
      日本農芸化学2016
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] Regulatory mechanism of TRP channel in yeast tonoplast2015

    • Author(s)
      Hamamoto, S., Yabe, I., and Uozumi, N.
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
    • Place of Presentation
      Honolulu Hawai,USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 大腸菌の陰イオン感受性変異株の単離と陰イオン耐性遺伝子の同定2015

    • Author(s)
      菅原慶太,早坂俊亮,浜本晋,井原邦夫,斎藤浩美,小林弘,魚住信之
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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