Modulation of organelle localized ion transporter by lipid molecule.
Project/Area Number |
15K18678
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
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Research Institution | Tohoku University |
Principal Investigator |
Hamamoto Shin 東北大学, 工学研究科, 助教 (10533812)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | イオンチャネル / 出芽酵母 / 液胞 / カルシウム / 酵母 / Ca2+ / トランスポーター / チャネル |
Outline of Final Research Achievements |
TRPY1 is a major cation channel localized in yeast vacuole membrane. We have applied the patch-clamp technique using enlarged yeast cells to elucidate the regulatory mechanisms of the TRPY1 activity. We have identified the Cys crucial for the functional property of the TRPY1 channel in the presence of reducing agent. Next we examined the effect of phosphatidyl inositol phosphate on TRPY1 function since there are several reports on the involvement of PI(3,5)P2 in hyperosmotic stress induced Ca2+ release from organelle. We assessed whether other phosphatidylinositol phosphates (PIPs) affect to the TRPY1 activity, which is monitored as aequorin-mediated luminescence in yeast mutants defective in phosphorylation and dephosphorylation of PIPs. One of mutant of PIP phosphatase showed decreased activity in hyperosmotic stress induced Ca2+ release. Furthermore, the patch-clamp analysis revealed that addition of PI(3)P to the cytoplasmic side reduced the whole-vacuole currents of TRPY1.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Identification and characterization of compounds that affect stomatal movements.2018
Author(s)
Toh S, Inoue S, Toda Y, Yuki T, Suzuki K, Hamamoto S, Fukatsu K, Aoki S, Uchida M, Asai E, Uozumi N, Sato A, Kinoshita T.
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Journal Title
Plant & Cell Physiology
Volume: in press
Issue: 8
Pages: 1568-1580
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] GTR1 is a jasmonic acid and jasmonoyl-l-isoleucine transporter in Arabidopsis thaliana.2017
Author(s)
Ishimaru, Y., Oikawa, T., Suzuki, T., Takeishi, S., Matsuura, H., Takahashi, K., Hamamoto, S., Uozumi, N., Shimizu, T., Seo, M., Ohta, H., and Ueda, M.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 81
Issue: 2
Pages: 249-255
DOI
Related Report
Peer Reviewed
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[Journal Article] The jasmonate-responsive GTR1 transporter is required for gibberellin-mediated stamen development in Arabidopsis2015
Author(s)
H. Saito, T. Oikawa, S. Hamamoto, Y. Ishimaru, T. Utsumi, J. Chen, M. Kanamori, Y. Sasaki-Sekimoto, M. Shimojima, S. Masuda, Y. Kamiya, M. Seo, N. Uozumi, M. Ueda, H. Ohta, A
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Journal Title
Nature Communications
Volume: 6
Issue: 1
Pages: 6095-6095
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Lipidation of Arabidopsis CPK6 promotes the plasma membrane targeting and the stomata closure mediated by activation of SLAC1 in Arabidopsis guard cells2016
Author(s)
Saito, S., Hamamoto, S., Sato, Y., Uozumi, N., Hashimoto, K., Kudla, J., Utsumi, T., Moriya, K., Tozawa, Y., and Yamauchi, S.
Organizer
International workshop on Plant Membrane Biology
Place of Presentation
Annapolis Maryland, USA
Year and Date
2016-06-05
Related Report
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[Presentation] Wound-inducible GTR1 transporter is involved in JA-Ile translocation during wound response in Arabidopsis2016
Author(s)
Oikawa,T., Ishimaru, Y., Takeishi, S., Matsuura, H., Takahashi, K., Hamamoto, S., Uozumi, N., Shimizu, T., Seo, M., Ohta, H., and Ueda., M.
Organizer
International workshop on Plant Membrane Biology
Place of Presentation
Annapolis Maryland, USA
Year and Date
2016-06-05
Related Report
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