Research Project
Grant-in-Aid for Young Scientists (B)
TRPY1 is a major cation channel localized in yeast vacuole membrane. We have applied the patch-clamp technique using enlarged yeast cells to elucidate the regulatory mechanisms of the TRPY1 activity. We have identified the Cys crucial for the functional property of the TRPY1 channel in the presence of reducing agent. Next we examined the effect of phosphatidyl inositol phosphate on TRPY1 function since there are several reports on the involvement of PI(3,5)P2 in hyperosmotic stress induced Ca2+ release from organelle. We assessed whether other phosphatidylinositol phosphates (PIPs) affect to the TRPY1 activity, which is monitored as aequorin-mediated luminescence in yeast mutants defective in phosphorylation and dephosphorylation of PIPs. One of mutant of PIP phosphatase showed decreased activity in hyperosmotic stress induced Ca2+ release. Furthermore, the patch-clamp analysis revealed that addition of PI(3)P to the cytoplasmic side reduced the whole-vacuole currents of TRPY1.
All 2018 2017 2016 2015
All Journal Article (8 results) (of which Int'l Joint Research: 2 results, Peer Reviewed: 8 results, Open Access: 6 results, Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 1 results)
Plant & Cell Physiology
Volume: in press Issue: 8 Pages: 1568-1580
10.1093/pcp/pcy061
New Phytologist
Volume: 218 Issue: 4 Pages: 1504-1521
10.1111/nph.15053
The Febs Journal
Volume: 285 Issue: 6 Pages: 1146-1161
10.1111/febs.14399
Plant Signaling & Behavior
Volume: 12 Issue: 6 Pages: 1334749-1334749
10.1080/15592324.2017.1334749
Scientific Reports
Volume: 7 Issue: 1 Pages: 1-9
10.1038/s41598-017-02164-7
Bioscience, Biotechnology, and Biochemistry
Volume: 81 Issue: 2 Pages: 249-255
10.1080/09168451.2016.1246174
Nature Communications
Volume: 6 Issue: 1 Pages: 6095-6095
10.1038/ncomms7095
Current Opinion in Biotechnology
Volume: 32 Pages: 113-120
10.1016/j.copbio.2014.11.025
