Algal protein kinase TAR1 modulates cellular viability and gametogenesis in carbon/nitrogen imbalance conditions
| Project/Area Number |
15K18682
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 植物生理学 / 植物分子生物学 / 脂質代謝 / 光合成 / 藻類育種 / タンパク質リン酸化 / 脂質 / デンプン / 栄養欠乏応答 / リン酸化プロテオーム / 藻類 / 藻類バイオテクノロジー / 炭素代謝 / 窒素代謝 |
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| Outline of Final Research Achievements |
When nutrient deprived, microalgae accumulate triacylglycerol (TAG) in lipid droplets. Dual-specificity kinase, TAG-accumulation-regulator-1 (TAR1) has been reported to regulate carbon metabolism in a green alga, Chlamydomonas reinhardtii. TAR1 is required for acetate-dependent TAG accumulation and degradation of chlorophyll and photosynthesis-related proteins in photomixotrophic nitrogen (N)-deficient conditions. We report that the tar1-1 mutant maintained high levels of cell viability and lower generation of H2O2 compared with wild-type (WT) cells in photoautotrophic N-deficient conditions. Mating efficiency and mRNA abundance of key regulators in gametogenesis were lower in tar1-1 than those in WT. In addition, tar1-1 accumulated higher levels of TAG and starch per cell volumes compared with WT. We identified total 426 TAR1-dependent phosphoproteins by phosphoproteomic analysis. Several protein kinases and enzymes related to N assimilation and carbon metabolism were included.
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Report
(4 results)
Research Products
(18 results)
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[Presentation] Isolation of green algal mutants accumulating high levels of TAG and chlorophyll under nitrogen deficient conditions2018
Author(s)
Asuka Miyamoto, Yuki Niikawa, Daisuke Shimamura, Tomoya Honjo, Noriko Kozai, Donghui Hu, Chihana Toyokawa, Haruka Shinkawa, Masataka Kajikawa, Takashi Yamano, Hideya Fukuzawa
Organizer
The 16th International Student Seminar
Related Report
Int'l Joint Research
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[Presentation] Identification of phosphorylation targets of TAR1 kinase, a regulator of lipid accumulation in C/N-stress conditions2017
Author(s)
Uran Ohta, Haruka Shinkawa, Masataka Kajikawa, Yuki Nomura, Mayu Ogura, Hirofumi Nakagami, Naoyuki Sugiyama, Yasushi Ishihama, Hideya Fukuzawa
Organizer
The 15th International Student Seminar
Place of Presentation
Kyoto university Shiran-kaikan, Kyoto
Year and Date
2017-02-23
Related Report
Int'l Joint Research
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