Structural basis of the self- and non-self-recognition in plant self-incompatibility

• Project/Area Number: 15K18683
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Murase Kohji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50467693)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 植物 / 構造生物学 / 相互作用 / タンパク質構造解析

Outline of Final Research Achievements

Many flowering plants have self-incompatibility for promotion of out-crossing to maintain the genomic diversity. In Brassica, pistil factor SRK and pollen factor SP11 coding in the S-locus control the self-incompatibility reaction. This pollen rejection is triggered by S-haplotype specific SRK-SP11 interaction. To study the mechanism of S-haplotype specific recognition of SP11 by SRK, we tried to structure determination of SRK-SP11 complex by X-ray crystallography. Here, he successfully expressed the recombinant SRK protein in insect cells, and obtain the large amount of purified SRK protein. We also obtained the protein crystals of SRK-SP11 complex. Our data will contribute to the determination of SRK-SP11 structure and understanding the mechanism of self-incompatibility in plants.

Research Products

• [Journal Article] Efficient expression of SRK intracellular domain by a modeling-based protein engineering. (2016)
  • Author(s): Murase K, Hirano Y, Takayama S, Hakoshima T.
  • Journal Title: Protein Expression and Purification Volume: 印刷中 Pages: 70-75
  • DOI: 10.1016/j.pep.2015.09.020
  • Peer Reviewed
• [Journal Article] Generation of transgenic <i>Linum perenne</i> by <i>Agrobacterium</i>-mediated transformation (2015)
  • Author(s): Murase, K., Sugai, Y., Hayashi, S., Suzuki, Y., Tsujii, K., Takayama, S.
  • Journal Title: Plant Biotechnology Volume: 32 Issue: 4 Pages: 349-352
  • DOI: 10.5511/plantbiotechnology.15.1109a
  • NAID: 130005115907
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed