| Project/Area Number |
15K18683
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Nara Institute of Science and Technology |
|---|
Principal Investigator |
Murase Kohji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50467693)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 植物 / 構造生物学 / 相互作用 / タンパク質構造解析 |
|---|
| Outline of Final Research Achievements |
Many flowering plants have self-incompatibility for promotion of out-crossing to maintain the genomic diversity. In Brassica, pistil factor SRK and pollen factor SP11 coding in the S-locus control the self-incompatibility reaction. This pollen rejection is triggered by S-haplotype specific SRK-SP11 interaction. To study the mechanism of S-haplotype specific recognition of SP11 by SRK, we tried to structure determination of SRK-SP11 complex by X-ray crystallography. Here, he successfully expressed the recombinant SRK protein in insect cells, and obtain the large amount of purified SRK protein. We also obtained the protein crystals of SRK-SP11 complex. Our data will contribute to the determination of SRK-SP11 structure and understanding the mechanism of self-incompatibility in plants.
|
