| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Branching enzyme (BE) catalyzes formation of branch points of glycogen and starch. Active site cleft (subsite) of BE has never been reported. In this study, crystal structures of BE1 from starch-producing cyanobacteria Cyanothece sp. ATCC 51142 in complex with linear maltooligosaccharides were determined. The crystal structures revealed the location of subsites and the presence of seven surface binding sites (SBSs). Importance of amino acid residues involved in sugar binding at subsites and SBSs in the crystal structures was confirmed by mutational analysis of the residues. In addition, our results suggest that two SBSs in the catalytic domain participate in binding of acceptor substrate. Based on these results, new detailed mechanistic model of BE reaction has been presented.
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