| Project/Area Number |
15K18686
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
Yasuda kaori 富山県立大学, 工学部, 助教 (70707231)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ビタミンD水酸化体 / シトクロムP450 / 骨粗鬆症 / P450 / 骨粗鬆症治療薬 |
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| Outline of Final Research Achievements |
There are lots of vitamin D hydroxylated compounds, and some of them are useful as medical drugs or authentic standard. In this study, we aimed to provide some vitamin D hydroxylated compounds such as active form of vitamin D2 by bioconversion process. We have reported the production of active form of vitamin D3 using the actinomycete CYP105A1 double variant, R73A/R84A. In this study, we found the new triple variant R73A/R84A/M239A to produce active form of vitamin D2. We also clarified that R73A/R84A was useful for the production of 25,26-dihydroxyvitamin D2, which is one of the vitamin D2 metabolite in the human body.
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