Production of vitamin D hydroxylated compounds
Project/Area Number |
15K18686
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
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Research Institution | Toyama Prefectural University |
Principal Investigator |
Yasuda kaori 富山県立大学, 工学部, 助教 (70707231)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | ビタミンD水酸化体 / シトクロムP450 / 骨粗鬆症 / P450 / 骨粗鬆症治療薬 |
Outline of Final Research Achievements |
There are lots of vitamin D hydroxylated compounds, and some of them are useful as medical drugs or authentic standard. In this study, we aimed to provide some vitamin D hydroxylated compounds such as active form of vitamin D2 by bioconversion process. We have reported the production of active form of vitamin D3 using the actinomycete CYP105A1 double variant, R73A/R84A. In this study, we found the new triple variant R73A/R84A/M239A to produce active form of vitamin D2. We also clarified that R73A/R84A was useful for the production of 25,26-dihydroxyvitamin D2, which is one of the vitamin D2 metabolite in the human body.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Production of an active form of vitamin D2 by genetically engineered CYP105A1.2017
Author(s)
K. Yasuda K, Y. Yogo , H.Sugimoto, H. Mano, T. Takita, M. Ohta, M. Kamakura, S. Ikushiro, K. Yasukawa, Y. Shiro, T. Sakaki.
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Journal Title
Biochem Biophys Res Commun
Volume: 486
Issue: 2
Pages: 336-341
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Production of an active form of vitamin D and drug metabolites by recombinant actinomycete expressing genetically engineered CYP105A1.2016
Author(s)
Yasuda K, Yogo Y, Takita T, Sugimoto H, Shiro Y, Mano H., Hayashi K, Kamakura M, Ohta M, lkushiro S, Yasukawa K, Sakaki T
Organizer
The Fifth International Conference on Cofactors and Active Enzyme Molecule 2016
Place of Presentation
Toyama
Year and Date
2016-09-04
Related Report
Int'l Joint Research
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[Presentation] Protein engineering of actinomycete CYP105A1 to produce active forms of vitamin D and drug metabolites2016
Author(s)
Yasuda K, Yogo Y, Takita T, Sugimoto H, Shiro Y, Mano H, Hayashi K, Kamakura M, Ohta M, lkushiro S, Yasukawa K, Sakaki T
Organizer
Cytochrome P450 Biodiversity & Biotechnology 2016
Place of Presentation
Vancouver
Year and Date
2016-07-22
Related Report
Int'l Joint Research / Invited
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[Presentation] Protein engineering of CYP105A1 to produce active forms of vitamin D and drug metabolites2015
Author(s)
Yuya Yogo, Kaori Yasuda, Teisuke Takita, Hiroshi Sugimoto, Yoshitsugu Shiro, Hiroki Mano, Keiko Hayashi, Masaki Kamakura, Miho Ohta, Shinichi lkushiro, Kiyoshi Yasukawa,
Organizer
The 19th International Conference on Cytochrome P450
Place of Presentation
東京
Year and Date
2015-06-12
Related Report
Int'l Joint Research
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