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Elucidation of phenylpropene biosynthetic pathway leading to aromatic compounds in plants and its application to metabolic engineering

Research Project

Project/Area Number 15K18690
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bioorganic chemistry
Research InstitutionYamaguchi University

Principal Investigator

Koeduka Takao  山口大学, 大学院創成科学研究科, 助教 (30565106)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords香気成分 / 二次代謝産物 / 二次代謝 / 二次代謝物
Outline of Final Research Achievements

In this study, we performed a screening of plants producing high levels of aromatic volatile compounds, phenylpropenes, and isolation of methylene-dioxy bridge forming enzymes and prenyltransferase, which are responsible for the diversification of phenylpropene structures. As the results, we found that dill, chervil, parsley, basil, illicium, and wild ginger produce high amounts of phenylpropenes including myristicine, dillapiole, and dimethylally eugenol. In addition, we successfully isolated putative genes encoding methylene-dioxy bridge forming enzymes and prenyltransferase from dill and illicium.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (20 results)

All 2018 2017 2016 2015

All Journal Article (11 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 11 results,  Open Access: 2 results,  Acknowledgement Compliant: 6 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Functional evolution of biosynthetic enzymes that produce plant volatiles.2018

    • Author(s)
      Koeduka, T.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 82 Issue: 2 Pages: 192-199

    • DOI

      10.1080/09168451.2017.1422968

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aromatic amino acid decarboxylase is involved in volatile phenylacetaldehyde production in loquat (<i>Eriobotrya japonica</i>) flowers2017

    • Author(s)
      Koeduka, T., Fujita, Y., Furuta, T., Suzuki, H., Tsuge, T. and Matsui, K.
    • Journal Title

      Plant Biotechnology

      Volume: 34 Issue: 4 Pages: 193-198

    • DOI

      10.5511/plantbiotechnology.17.0926a

    • NAID

      130006321254

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An improved synthesis of the potent and selective γ-glutamyl transpeptidase inhibitor GGsTop together with an inhibitory activity evaluation of its potential hydrolysis products.2017

    • Author(s)
      Watanabe, B., Morikita, T., Tabuchi, Y., Kobayashi, R., Koeduka, T. and Hiratake, J.
    • Journal Title

      Tetrahedron Letters

      Volume: 58 Issue: 38 Pages: 3700-3703

    • DOI

      10.1016/j.tetlet.2017.08.019

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An adenylyl cyclase with a phosphodiesterase domain in basal plants with a motile sperm system2017

    • Author(s)
      Kasahara, M., Suetsugu, N., Urano, Y., Yamamoto, C., Ohmori, M., Takada, Y., Okuda, S., Nishiyama, T., Sakayama, H., Kohchi, T., and Takahashi, F.
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 6 Pages: 39232-39232

    • DOI

      10.1080/09168451.2017.1285688

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biosynthesis of volatile terpenes that accumulate in the secretory cavities of young leaves of Japanese pepper (<i>Zanthoxylum piperitum</i>): Isolation and functional characterization of monoterpene and sesquiterpene synthase genes2017

    • Author(s)
      Fujita, Y., Koeduka, T., Aida, M., Suzuki, H., Iijima, Y., & Matsui, K.
    • Journal Title

      Plant Biotechnology

      Volume: 34 Issue: 1 Pages: 17-28

    • DOI

      10.5511/plantbiotechnology.16.1231a

    • NAID

      130005519607

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Acrolein-detoxifying isozymes of glutathione transferase in plants.2017

    • Author(s)
      Mano, J., Ishibashi, A., Muneuchi, H., Morita, C., Sakai, H., Biswas, Md. S., Koeduka, T. and Kitajima, S.
    • Journal Title

      Planta

      Volume: 245 Issue: 2 Pages: 255-264

    • DOI

      10.1007/s00425-016-2604-5

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Characterization of an O-methyltransferase specific to guaiacol-type benzenoids from the flowers of loquat (Eriobotrya japonica)2016

    • Author(s)
      Koeduka, T., Kajiyama, M., Furuta, T., Suzuki, H., Tsuge, T. and Matsui, K.
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 122 Issue: 6 Pages: 679-684

    • DOI

      10.1016/j.jbiosc.2016.06.012

    • NAID

      40021044912

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Direct evidence of specific localization of sesquiterpenes and marchantin A in oil body cells of Marchantia polymorpha L..2016

    • Author(s)
      Tanaka, M., Esaki, T., Kenmoku, H., Koeduka, K., Kiyoyama, Y., Masujima, T., Asakawa, Y. and Matsui, K.
    • Journal Title

      Phytochemistry

      Volume: 130 Pages: 77-84

    • DOI

      10.1016/j.phytochem.2016.06.008

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Benzenoid biosynthesis in the flowers of Eriobotrya japonica: molecular cloning and functional characterization of p-methoxybenzoic acid carboxyl methyltransferase.2016

    • Author(s)
      Koeduka, T., Kajiyama, M., Suzuki, H., Furuta, T., Tsuge, T. and Matsui, K.
    • Journal Title

      Planta

      Volume: 244 Issue: 3 Pages: 725-736

    • DOI

      10.1007/s00425-016-2542-2

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Arabidopsis lipoxygenase 2 is essential for formation of green leaf volatiles and five-carbon volatiles2016

    • Author(s)
      S. Mochizuki, K. Sugimoto, T. Koeduka, K. Matsui
    • Journal Title

      FEBS Letters

      Volume: 590 Issue: 7 Pages: 1017-1027

    • DOI

      10.1002/1873-3468.12133

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Biochemical characterization of allene oxide syntheses from the liverwort Marchantia polymorphs and green micro algae klebsormidium flaccid provides insight into the evolutionary divergence of the plant CYP74 family2015

    • Author(s)
      Koeduka T, Ishizaki K, Mwenda CM, Hori K, Sasaki-Sekimoto Y, Ohta H, Kohchi T, Matsui K
    • Journal Title

      Planta

      Volume: 242 Issue: 5 Pages: 427-443

    • DOI

      10.1007/s00425-015-2355-8

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Biosynthesis of volatile terpenes that accumulate in the secretory cavities of young Japanese pepper (Zanthoxylum piperitum) leaves.2018

    • Author(s)
      Koeduka T, Fujita Y, Takaishi M, Aida M, Iijima Y, Matsui K
    • Organizer
      Gordon Research Conferences-Plant Volatiles
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] サンショウ油胞特異的に蓄積する揮発性テルペンの生成に関する研究2017

    • Author(s)
      肥塚崇男、藤田芳勧、飯島陽子、相田光宏、鈴木秀幸、松井健二
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都府京都市 京都女子大学
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] ショウブ由来レスベラトロールO-メチル基転移酵素の機能解析2017

    • Author(s)
      肥塚崇男, 畑田珠希, 鈴木秀幸, 鈴木史朗, 松井健二
    • Organizer
      第35回日本植物細胞分子生物学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物の進化とみどりの香り生成能の獲得2017

    • Author(s)
      肥塚崇男, 松井健二
    • Organizer
      イメージング研究会 第九回光塾
    • Related Report
      2017 Annual Research Report
  • [Presentation] フェニルプロペン香気成分の生合成に関わるモノリグノールアセチル化酵素遺伝子の単離と機能解析2016

    • Author(s)
      肥塚崇男、鈴木史朗、飛松裕基、宮本託志、梅澤俊明、松井健二
    • Organizer
      第61回 リグニン討論会
    • Place of Presentation
      京都府宇治市 京都大学
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
  • [Presentation] ビワの揮発性ベンゼノイド生合成に関与するカルボン酸メチル化酵素の機能解析2016

    • Author(s)
      肥塚崇男、梶山眞未、望月智史、古田巧、鈴木秀幸、柘植知彦、松井健二
    • Organizer
      第34回日本植物細胞分子生物学会
    • Place of Presentation
      長野県上田市 信州大学
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 植物香気成分の多様性と生合成酵素の機能進化2016

    • Author(s)
      肥塚崇男
    • Organizer
      日本農芸化学会中四国支部 第23回若手シンポジウム(第8回農芸化学の未来開拓セミナー)
    • Place of Presentation
      岡山県岡山市 岡山大学
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] The origin and evolution of plant CYP74 superfamily involved in oxylipin biosynthesis2015

    • Author(s)
      Koeduka T, Ishizaki K, Mwenda CM, Hori K, Nishihama R, Sasaki-Sekimoto Y, Ohta H, Kohchi T, Matsui K
    • Organizer
      6th Asian Symposium on Plant Lipids
    • Place of Presentation
      シンガポール、シンガポール共和国
    • Year and Date
      2015-12-02
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 脂肪酸ヒドロペルオキシドを基質とするCYP74ファミリーの分子進化2015

    • Author(s)
      肥塚崇男、石崎公庸、Cynthia Mugo Mwenda、堀孝一、佐々木(関本)結子、太田啓之、河内孝之、松井健二
    • Organizer
      第33回 植物分子細胞生物学会
    • Place of Presentation
      東京都文京区 東京大学弥生キャンパス
    • Year and Date
      2015-08-09
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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