| Project/Area Number |
15K18699
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TAMURA Hirotoshi 香川大学, 農学部, 教授 (00188442)
GOHTANI Shoichi 香川大学, 農学部, 教授 (00153742)
MATSUMOTO Yoshiki 香川大学, 農学部, 准教授 (90335844)
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| Research Collaborator |
NAMBA Go
IDEGAMI Nao
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Caco-2 / HT29-MTX / permeability assay / mucus layer / carotenoids / permeability / bioavailability / assay / HT29/MTX / phytochemicals / permeabiity assay / カロテノイド / 消化 / 吸収 / 食品 |
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| Outline of Final Research Achievements |
Although Caco-2 cell-based permeation assays are used to predict the oral absorption of drugs and phytochemicals in humans, they lack an important feature of the human intestine: the mucus layer on the luminal surface. We have developed mucus-layered cell model using Caco-2 combined with the mucus-secreting HT29-MTX cell-line. We confirmed the presence of a mucus layer on top of Caco-2/HT28-MTX monolayers by scanning electron microscopy and fluorescence microscopy. β-carotene uptake in the mucus-layered Caco-2/HT29-MTX model was clearly lower than in the standard model when β-carotene was carried by larger micelles, but not when carried by very small PC micelles, suggesting that the mucus layer inhibited β-carotene uptake by intestinal cells, probably by slowing the permeation of larger mixed micelles. The new mucus-layered model could also effectively detect the enhancement of β-carotene absorption by certain phospholipids.
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