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Elucidation of molecular mechanisms for function control of mast cells aiming at construction of anti-allergy method by food

Research Project

Project/Area Number 15K18702
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Food science
Research InstitutionTokyo University of Science

Principal Investigator

Kasakura Kazumi  東京理科大学, 基礎工学部生物工学科, 日本学術振興会特別研究員(PD) (00724577)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsマスト細胞 / アレルギー / 転写因子 / 食品成分
Outline of Final Research Achievements

① In the current study, involvement of the transcription factors, PU.1, GATA1 and GATA2 in the expression of allergy related genes in mast cells was analyzed. It was revealed that knockdown of PU.1 or GATA2 decreased FcεRI expression and suppressed the IgE-mediated activation of mast cells. In addition, Syk was identified as a new target gene of PU.1. ② We found that Tannic acid decreased surface FcεRI expression via silencing of GATA2. Moreover, oral administration of Tannic acid inhibited IgE/Ag-induced passive systemic anaphylaxis. Collectively, these results demonstrate that transcription factors that regulate mast cell-specific gene expression are targets for prevention of mast cell-mediated allergic diseases and that food ingredients could be an effective tool.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (28 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (25 results)

  • [Journal Article] The hematopoietic cell-specific transcription factor PU.1 is critical for expression of CD11c.2017

    • Author(s)
      Yashiro T, Kasakura K, Oda Y, Kitamura N, Inoue A, Nakamura S, Yokoyama H, Fukuyama K, Hara M, Ogawa H, Okumura K, Nishoiyama M, Nishoiyama C
    • Journal Title

      International immunology

      Volume: 29 Issue: 2 Pages: 87-94

    • DOI

      10.1093/intimm/dxx009

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Role of PU.1 in MHC class II Expression via CIITA Transcription in Plasmacytoid Dendritic Cells2016

    • Author(s)
      Miura R, Kasakura K, Nakano N, Hara M, Maeda K, Okumura K, Ogawa H, Yashiro T, Nishiyama C
    • Journal Title

      PLOS ONE

      Volume: 11 Issue: 4 Pages: e0154094-e0154094

    • DOI

      10.1371/journal.pone.0154094

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of PU.1 in NFATc1 promoter function in osteoclast development2015

    • Author(s)
      Ishiyama K, Yashiro T, Nakano N, Kasakura K, Miura R, Hara M, Kawai F, Maeda K, Tamura N, Okumura K, Ogawa H, Takasaki Y, Nishiyama C
    • Journal Title

      Allergology International

      Volume: 64 Issue: 3 Pages: 241-247

    • DOI

      10.1016/j.alit.2015.01.006

    • NAID

      130005290298

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] GATA2によるマスト細胞プロテアーゼの発現制御機構2017

    • Author(s)
      笠倉和巳、中谷光、新井貴大、八代拓也、西山千春
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] マスト細胞活性化反応に対する短鎖脂肪酸の抑制効果2017

    • Author(s)
      藤垣泉、笠倉和巳、八代拓也、西山千春
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規非タンパク性アミノ酸DADHによる免疫抑制作用2017

    • Author(s)
      関本崇宏、八代拓也、長谷部文人、松田研一、笠倉和巳、西山真、西山千春
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規アミノ酸DADHによるマスト細胞の活性抑制2017

    • Author(s)
      白井智丈、笠倉和巳、長谷部文人、松田研一、八代拓也、西山真、西山千春
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 転写因子PU.1のアレルギー疾患治療標的としての有効性2017

    • Author(s)
      小田祥人、笠倉和巳、八代拓也、西山千春
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] The nuclear orphan receptor NR4a3/NOR1 is involved in the function of dendritic cells.2016

    • Author(s)
      YASHIRO Takuya、KASAKURA Kazumi、NISHIYAMA Chiharu
    • Organizer
      日本免疫学会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2016-12-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] GATA2 is involved in the expression of the decoy receptor for IL-33 by binding to the proximal promoter with chromosomal loop formation2016

    • Author(s)
      KASAKURA Kazumi、YASHIRO Takuya、HARA Mutsuko、OKUMURA Ko、NISHIYAMA Chiharu
    • Organizer
      日本免疫学会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2016-12-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] インフラマソーム構成分子NLRP3遺伝子の単球系細胞特異的発現制御機構の解明2016

    • Author(s)
      山本満智子、八代拓也、笠倉和巳、西山千春
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] NR4a3によるマクロファージにおけるMDC/Ccl22発現調節2016

    • Author(s)
      内田佑奈、八代拓也、笠倉和巳、西山千春
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 樹状細胞におけるPD-L2の遺伝子発現制御機構の解析2016

    • Author(s)
      渡辺良介、八代拓也、笠倉和巳、西山千春
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] マスト細胞における転写因子Ehfの機能解析2016

    • Author(s)
      内田万紀子、中野信浩、原むつ子、笠倉和巳、八代拓也、西山千春
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] PU.1を標的としたマスト細胞依存性アレルギー反応の抑制2016

    • Author(s)
      小田祥人、笠倉和巳、八代拓也、西山千春
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 短鎖脂肪酸によるマスト細胞の機能制御2016

    • Author(s)
      藤垣泉、笠倉和巳、八代拓也、西山千春
    • Organizer
      日本食品免疫学会
    • Place of Presentation
      東京大学
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Tannic acidによるアレルギー抑制効果とそのメカニズム解析2016

    • Author(s)
      濱田他琢人、笠倉和巳、八代拓也、西山千春
    • Organizer
      日本食品免疫学会
    • Place of Presentation
      東京大学
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 樹状細胞の免疫調節機能に対するPterostilbeneの作用2016

    • Author(s)
      由良志織、八代拓也、笠倉和巳、西山千春
    • Organizer
      日本食品免疫学会
    • Place of Presentation
      東京大学
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウスIgE抗体への結合活性が上昇したヒトFcεRIαサブユニット改変体の作製2016

    • Author(s)
      山本真也, 中野信浩, 八代拓也, 笠倉和巳, 奥村康, 西山真, 西山千春
    • Organizer
      日本農芸化学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 樹状細胞の活性化反応における核内受容体型転写因子NR4a3の機能解析2016

    • Author(s)
      八代拓也, 長岡雅典, 笠倉和巳, 西山千春
    • Organizer
      日本農芸化学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 樹状細胞においてRALDH2発現を亢進する食品成分の探索2016

    • Author(s)
      山口昌樹, 八代拓也, 笠倉和巳, 西山千春
    • Organizer
      日本農芸化学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] マスト細胞におけるC/EBPα遺伝子の発現抑制の調節2016

    • Author(s)
      笠倉和巳, 八代拓也, 原むつ子, 奥村康, 西山千春
    • Organizer
      日本農芸化学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] T細胞におけるCCR7発現制御機構の解析2015

    • Author(s)
      竹内裕美, 八代拓也, 笠倉和巳, 西山千春
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Cd207遺伝子は転写調節因子PU.1によって転写制御される2015

    • Author(s)
      中村秀輔, 八代拓也, 笠倉和巳, 西山千春
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] マスト細胞におけるIL-10発現制御機構の解明2015

    • Author(s)
      太田彩花, 笠倉和巳, 八代拓也, 西山千春
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 樹状細胞におけるRALDH2の発現制御機構2015

    • Author(s)
      山口昌樹, 八代拓也, 笠倉和巳, 西山千春
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] PU.1 regulates expression of the Syk gene in mast cells2015

    • Author(s)
      ODA Yoshito, KASAKURA Kazumi, YASHIRO Takuya, OKUMURA Ko, NISHIYAMA Chiharu
    • Organizer
      日本免疫学会
    • Place of Presentation
      札幌
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 7)PU.1 regulates MHC class II expression by transactivation of CIITA in plasmacytoid dendritic cells2015

    • Author(s)
      MIURA Ryosuke, KASAKURA Kazumi, NAKANO Nobuhiro, YASHIRO Takuya, NISHIYAMA Chiharu
    • Organizer
      日本免疫学会
    • Place of Presentation
      札幌
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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