| Project/Area Number |
15K18724
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Wood science
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
Shigeto Jun 九州大学, 農学研究院, 学術研究員 (70570852)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 植物ペルオキシダーゼ / リグニン / dehydrogenative polymer / DHPs |
|---|
| Outline of Final Research Achievements |
Ligninfication-related Arabidopsis peroxidases (AtPrx2, 25 and 71) and the poplar cationic cell-wall-bound peroxidase (CWPO-C) have an ability to oxidize lignin monomer model compounds. In this study, I tried to produce dehydrogenative polymers (DHPs) from lignin monomer to evaluate the lignin producing potential of these peroxidases, using the recombinant (rec) proteins. My results showed recAtPrx2, 25, 71 and recCWPO-C were able to produce DHPs from both guaiacyl and syringyl type of lignin monomer. In addition, the use of recCWPO-C enabled production of the syringyl type DHPs, with similar distribution of molecular size to milled wood lignin. These results suggested that plant peroxidases involved in lignification have a superior property in the ability to polymerize syringyl type lignin monomer and enlarge molecule size, rather than the plant peroxidases which do not involved in lignification.
|
