Development of tenacibaculosis vaccine

• Project/Area Number: 15K18732
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: Kusumoto Akiko 帯広畜産大学, 動物・食品検査診断センター, 助教 (60535326)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 滑走細菌 / ワクチン / 滑走運動 / 次世代シークエンサー / 魚病 / 9型輸送装置 / バクテロイデス / 接着

Outline of Final Research Achievements

Tenacibaculum maritimum is the causative agent of tenacibaculosis which affects a large number of marine fish species and is characterized by fin and mouth erosion, and necrotic ulcers of skin. In Japan, there is no available tenacibaculosis vaccine for cultured fish. Use of medicine for this disease is limited. To develop tenacibaculosis vaccine, we focused on gliding motility of T. maritimum, since bacterial motility is involved in virulence in many pathogenic bacteria. We generated mutants showing abnormal gliding motility by UV irradiation, and analyzed them by next-generation sequencer.